RT Journal Article
T1 Changes in Intestinal Microbiota and Predicted Metabolic Pathways During Colonic Fermentation of Mango (Mangifera indica L.)—Based Bar Indigestible Fraction
A1 Gutiérrez Sarmiento, Wilbert
A1 Sáyago Ayerdi, Sonia Guadalupe
A1 Goñi Cambrodón, Isabel
A1 Gutiérrez Miceli, Federico Antonio
A1 Abud-Archila, Miguel
A1 Rejón Orantes, José del Carmen
A1 Rincón Rosales, Reiner
A1 Peña Ocaña, Betsy Anaid
A1 Ruíz Valdiviezo, Víctor Manuel
AB Mango (Mangifera indica L.) peel and pulp are a source of dietary fiber (DF) and phenolic compounds (PCs) that constituent part of the indigestible fraction (IF). This fraction reaches the colon and acts as a carbon and energy source for intestinal microbiota. The effect of mango IF on intestinal microbiota during colonic fermentation is unknown. In this study, the isolated IF of a novel ‘Ataulfo’ mango-based bar (snack) UV-C irradiated and non irradiated (UVMangoBand MangoB) were fermented. Colonic fermentation occurred in vitro under chemical-enzymatic, semi-anaerobic, batch culture and controlled pH colonic conditions. Changes in the structure of fecal microbiota were analyzed by 16s rRNA gene Illumina MiSeq sequencing. The community´s functional capabilities were determined in silico. The MangoB and UVMangoB increased the presence of Faecalibacterium, Roseburia, Eubacterium, Fusicatenibacter, Holdemanella, Catenibacterium, Phascolarctobacterium, Buttiauxella, Bifidobacterium, Collinsella, Prevotella and Bacteroides genera. The alpha indexes showed a decrease in microbial diversity after 6 h of colonic fermentation. The coordinates analysis indicated any differences between irradiated and non-irradiated bar. The metabolic prediction demonstrated that MangoB and UVMangoB increase the microbiota carbohydrate metabolism pathway. This study suggests that IF of mango-based bar induced beneficial changes on microbial ecology and metabolic pathway that could be promissory to prevention or treatment of metabolic dysbiosis. However, in vivo interventions are necessary to confirm the interactions between microbiota modulating and intestinal beneficial effects.
PB MDPI
SN 2072-6643
YR 2020
FD 2020-03-03
LK https://hdl.handle.net/20.500.14352/8651
UL https://hdl.handle.net/20.500.14352/8651
LA eng
NO Consejo Nacional de Ciencia y Tecnología (CONACYT), Mexico
NO Tecnológico Nacional de México (TECNM)
DS Docta Complutense
RD 10 abr 2025