RT Journal Article
T1 Complexation of Hg with phytochelatins is important for plant Hg tolerance
A1 Carrasco Gil, Sandra
A1 Álvarez Fernández, Ana
A1 Sobrino Plata, Juan
A1 Millán, Rocío
A1 Carpena Ruiz, Ramón
A1 Leduc, Danika
A1 Andrews, Joy
A1 Abadía, Javier
A1 Hernández, Luís
AB Three-week-old alfalfa (Medicago sativa), barley (Hordeum vulgare) and maize (Zea mays) were exposed for 7 d to 30 µm of mercury (HgCl2) to characterize the Hg speciation in root, with no symptoms of being poisoned. The largest pool (99%) was associated with the particulate fraction, whereas the soluble fraction (SF) accounted for a minor proportion (<1%). Liquid chromatography coupled with electro-spray/time of flight mass spectrometry showed that Hg was bound to an array of phytochelatins (PCs) in root SF, which was particularly varied in alfalfa (eight ligands and five stoichiometries), a species that also accumulated homophytochelatins. Spatial localization of Hg in alfalfa roots by microprobe synchrotron X-ray fluorescence spectroscopy showed that most of the Hg co-localized with sulphur in the vascular cylinder. Extended X-ray Absorption Fine Structure (EXAFS) fingerprint fitting revealed that Hg was bound in vivo to organic-S compounds, i.e. biomolecules containing cysteine. Albeit a minor proportion of total Hg, Hg–PCs complexes in the SF might be important for tolerance to Hg, as was found with Arabidopsis thaliana mutants cad2-1 (with low glutathione content) and cad1-3 (unable to synthesize PCs) in comparison with wild type plants. Interestingly, high-performance liquid chromatography-electrospray ionization-time of flight analysis showed that none of these mutants accumulated Hg–biothiol complexes.
PB Wiley
SN 0140-7791
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/99571
UL https://hdl.handle.net/20.500.14352/99571
LA eng
NO Carrasco‐Gil, Sandra, et al. «Complexation of Hg with Phytochelatins Is Important for Plant Hg Tolerance». Plant, Cell & Environment, vol. 34, n.o 5, mayo de 2011, pp. 778-91. https://doi.org/10.1111/j.1365-3040.2011.02281.x.
NO Fundación Ramón Areces
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
NO Junta de Comunidades Castilla-La Mancha
NO Gobierno de Aragón
DS Docta Complutense
RD 6 abr 2025