RT Generic
T1 Nuevos recubrimientos biocidas en implantología. Evaluación, mediante un biofilm formado por 6 tipos de bacterias subgingivale
A1 Pianeta Alviz, Roquelina del Rosario
AB Antecedentes y objetivos: La biopelícula oral ocupa un lugarimportantísimo en el desarrollo de las enfermedades periimplantarias (mucositis o periimplantitis). En laactualidad, se sabe muy poco sobre el comportamiento de estas comunidades bacterianas periimplantarias al ser sometidas a recubrimientos biocidas sobre implantes, pilaresy demás aditamentos implantológicos que puedan estar encontacto con ellas. El objetivo de esta investigación fueobservar el comportamiento de un material compuesto cerámica-­cerámica con recubrimiento con vidrio biocida, sobre un modelo de biofilm in vitro que contiene seis bacterias de la microbiota subgingival, puesto a punto en elLaboratorio de Investigación de la Facultad de Odontologíade la Universidad Complutense de MadridMaterial y método: Se emplearon seis cepas de referencia para desarrollar biofilms sobre la superficie de discos dehidroxiapatita (HA) de 7 mm de diámetro y 1.8 mm de espesor y sobre discos de Nano Composite Z10Ce, con recubrimiento con corindón blanco, recubiertos con vidrio CINN (materialA2). Se depositaron tres discos de cada material en placasestériles de cultivo celular de 24 pocillos y se cubrieroncon 1.5 mL del inóculo (en medio BHI modificado), preparadocon el total de las bacterias y se incubaron en anaerobiosisdurante 24h, 48h y 72h a 37ºC. Las especies seleccionadas representan colonizadores iniciales (Streptococcus oralis yActinomyces naeslundii), tempranos (Veillonella parvula), secundarios (Fusobacterium nucleatum) y tardíos (Porphyromonas gingivalis y Aggregatibacter actinomycetemcomitans). El análisis de viabilidad bacterianaen el biofilm se realizó mediante Microscopía Confocal (CLSM). El número de unidades formadoras de colonias (UFC)/mL de cada especie presentes en el biofilm se obtuvomediante recuento visual con microscopio óptico. Para elanálisis estadístico, se utilizó un test estadístico de comparación de medias (t test) y el análisis de la varianza(ANOVA).Resultados: En el presente estudio, según el análisis estadístico, se encontraron pocas diferencias estadísticamente significativas entre el número de bacteriasque participan en el desarrollo del biofilm in vitro sobrehidroxiapatita (HA) y el material A2. Aunque, según el recuento bacteriano por cultivo, se pudieron observar mayores diferencias entre ambos materiales Estas diferenciasse confirman con los resultados de la microscopía láserconfocal (CLSM).Conclusiones: De forma preliminar, este estudio demuestraque el Nano Composite Z10Ce, recubierto con vidrio CINN(material A2), parece que ejerce un efecto biocida en eldesarrollo de un modelo de biofilm subgingival in vitro, aunque los datos estadísticos no revelan diferencias significativas. Deben llevarse a cabo más experimentos paracomprobar los resultados mostrados en este estudio.
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/36440
UL https://hdl.handle.net/20.500.14352/36440
LA spa
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DS Docta Complutense
RD 28 abr 2024