RT Journal Article
T1 Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level
A1 Pérez Sancho, Marta
A1 Cerdá, Ignacio
A1 Fernández-Bravo, Ana
A1 Domínguez Rodríguez, Lucas José
A1 Figueras, María José
A1 Fernández-Garayzábal Fernández, José Francisco
A1 Vela Alonso, Ana Isabel
AB The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options, the only species that showed values >90% was A. hydrophila. Overall, when the database was supplemented with 14 new spectra, the number of accurate identifications increased (41% vs. 55%) and the number of inconclusive identifications decreased (45% vs. 29%), but great differences in the success of species-level identifications were found. Species-distinctive mass peaks were identified only for A. hydrophila and A. bestiarum (5003 and 7360 m/z in 95.5% and 94.1% of their isolates, respectively). This work demonstrates the utility of MALDI-TOF MS for Aeromonas identification to the genus level, but there is no consistency for the accurate identification of some of the most prevalent species implicated in fish disease.
PB Wiley
SN 0140-7775
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/94088
UL https://hdl.handle.net/20.500.14352/94088
LA eng
NO Perez-Sancho M, Cerda I, Fern andez Bravo A, et al. Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level. J Fish Dis. 2018;41:1485–1493. https://doi.org/10.1111/jfd.12837
NO Comunidad de Madrid
NO European Commission
DS Docta Complutense
RD 16 abr 2025