RT Journal Article
T1 Electrochemical immunosensing of Growth arrest-specific 6in human plasma and tumor cell secretomes
A1 Muñoz San Martín, Cristina
A1 Pérez Ginés, Víctor
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Gamella Carballo, María
A1 Solís Fernández, Guillermo
A1 Montero Calle, Ana
A1 Pedrero Muñoz, María
A1 Serafín González-Carrato, Verónica
A1 Martínez Bosch, Neus
A1 Navarro, Pilar
A1 García de Frutos, Pablo
A1 Batlle, Montserrat
A1 Barderas Manchado, Rodrigo
A1 Pingarrón Carrazón, José Manuel
A1 Campuzano Ruiz, Susana
AB Growth arrest-specific 6 (GAS6) protein plays a key role in processes related to proliferation, inflammation, angiogenesis, and atherosclerotic plaque formation. In addition, it has been reported that plasma levels of GAS6 are related to cancer prognosis and other relevant pathologies, such as heart failure or sepsis. We report here the first electrochemical immunoplatform for the determination of GAS6, which has demonstrated to be competitive with other available methodologies in terms of cost, simplicity, and decentralized application. The developed immunoplatform involves a sandwich immunoassay using magnetic microparticles (MBs) and uses amperometric detection at disposable screen-printed carbon electrodes (SPCEs). The MBs were modified with an antibody specific to GAS6 for its selective capture, which is further recognized by a biotinylated secondary antibody subsequently labeled with a streptavidin-horseradish peroxidase (Strep-HRP) conjugate. The electrochemical detection was carried out using the hydroquinone (HQ)/H2O2 system. The developed bioplatform exhibits a great selectivity and low limit of detection (27 pg/mL) that allowed the determination of the GAS6 circulating level in plasma samples from patients suffering heart failure (HF) and diagnosed with pancreatic ductal adenocarcinoma (PDAC), as well as the determination of the target protein in raw secretomes of human colorectal cancer cell lines.
PB Wiley
YR 2021
FD 2021-07-09
LK https://hdl.handle.net/20.500.14352/132184
UL https://hdl.handle.net/20.500.14352/132184
LA eng
NO Muñoz‐San Martín, Cristina, et al. «Electrochemical Immunosensing of Growth Arrest‐specific 6 in Human Plasma and Tumor Cell Secretomes». Electrochemical Science Advances, vol. 2, n.o 4, agosto de 2022, p. e2100096. DOI.org (Crossref), https://doi.org/10.1002/elsa.202100096.
NO Ministerio de Ciencia e Innovación
NO Comunidad de Madrid
NO Ministerio de Educación, Cultura y Deporte
NO Complutense University of Madrid
DS Docta Complutense
RD 21 mar 2026