RT Journal Article
T1 Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages
A1 Rodríguez-Rubio, Lorena
A1 Serna Bernaldo, Carlos
A1 Ares Arroyo, Manuel
A1 Matamoros, Bosco R
A1 Delgado Blas, José Francisco
A1 Montero Serra, Natalia
A1 Bernabe-Balas, Cristina
A1 Wedel, Emilia Florentine
A1 Sánchez Méndez, Irene
A1 Muniesa, Maite
A1 González Zorn, Bruno
AB ObjectivesTo investigate the relevance of multicopy plasmids in antimicrobial resistance and assess their mobilization mediated by phage particlesMethodsSeveral databases with complete sequences of plasmids and annotated genes were analysed. The 16S methyltransferase gene armA conferring high-level aminoglycoside resistance was used as a marker in eight different plasmids, from different incompatibility groups, and with differing sizes and plasmid copy numbers. All plasmids were transformed into Escherichia coli bearing one of four different lysogenic phages. Upon induction, encapsidation of armA in phage particles was evaluated using qRT–PCR and Southern blotting.ResultsMulticopy plasmids carry a vast set of emerging clinically important antimicrobial resistance genes. However, 60% of these plasmids do not bear mobility (MOB) genes. When carried on these multicopy plasmids, mobilization of a marker gene armA into phage capsids was up to 10000 times more frequent than when it was encoded by a large plasmid with a low copy number.ConclusionsMulticopy plasmids and phages, two major mobile genetic elements (MGE) in bacteria, represent a novel high-efficiency transmission route of antimicrobial resistance genes that deserves further investigation.
PB Oxford University Press
SN 0305-7453
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/130361
UL https://hdl.handle.net/20.500.14352/130361
LA eng
NO Lorena Rodríguez-Rubio, Carlos Serna, Manuel Ares-Arroyo, Bosco R Matamoros, Jose F Delgado-Blas, Natalia Montero, Cristina Bernabe-Balas, Emilia F Wedel, Irene S Mendez, Maite Muniesa, Bruno Gonzalez-Zorn, Extensive antimicrobial resistance mobilization via multicopy plasmid encapsidation mediated by temperate phages, Journal of Antimicrobial Chemotherapy, Volume 75, Issue 11, November 2020, Pages 3173–3180, https://doi.org/10.1093/jac/dkaa311
NO European Commission
NO Ministerio de Innovación y Ciencia (España)
NO Generalitat de Catalunya
NO Centre de Referència en Biotecnologia
NO Ministerio de Educación (España)
NO Ministerio de Economía (España)
DS Docta Complutense
RD 18 mar 2026