RT Journal Article
T1 Equivalent seminal characteristics in human and stallion at first and second ejaculated fractions
A1 De la Torre, Javier
A1 Sánchez Martín, Pascual
A1 Gosálvez Berenguer, Jaime
A1 Crespo Castejón, Francisco
AB Sperm quality was assessed in normozoospermic human (n = 10) and Spanish breed stallion (n = 10) after sperm fractionation during ejaculation. The first ejaculated frac-tion was separated from the second. A third sample was reconstituted using equiva-lent proportion of both fractions (RAW). Fraction 1, Fraction 2 and RAW semen were incubated for 30 min at 37°C to homogenise the impact of iatrogenic damage be-tween both species. Sperm concentration, motility and sperm DNA damage were as-sessed in each fraction and RAW semen. The results showed two important facts: (i) spermatozoa confined at Fraction 1 exhibit superior parameters than those included at Fraction 2 in both species, and (ii) there is a certain level of concordance between spe-cies in the proportion of benefit observed when Fraction 1 is compared to RAW semen. Altogether, these results call into question whether the standard practice of whole ejaculate collection can be considered the best strategy when using male gam-etes for artificial insemination. In fact, the reconstituted RAW semen exhibits poorer semen characteristics than those found in Fraction 1.
PB Wiley
SN 0303-4569
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/97392
UL https://hdl.handle.net/20.500.14352/97392
LA eng
NO de la Torre J, Sánchez-Martín P, Gosálvez J, Crespo F. Equivalent seminal characteristics in human and stallion at first and second ejaculated fractions. Andrologia. 2017 Oct;49(8)
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 4 abr 2025