RT Journal Article
T1 Nanocellulose-modified electrodes for simultaneous biosensing of microbiome-related oral diseases biomarkers
A1 Ramos López, Claudia
A1 García Rodrigo, Lorena
A1 Sánchez Tirado, Esther
A1 González Cortés, Araceli
A1 Agüí Chicharro, María Lourdes
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB The preparation of a dual immunosensor for the simultaneous determination of two important microbiome-related oral disease biomarker matrix metalloproteinases MMP-9 and MMP-13 is reported. Screen-printed dual carbon electrodes (SPdCEs) modified with reduced graphene oxide and crystalline nanocellulose (rGO/CNC) were used for the immobilization of specific capture antibodies and the implementation of sandwich-type immunoassays using biotinylated secondary antibodies. Amperometric detection through the hydroquinone HQ/H2O2 system was employed. The limits of detection (LOD) achieved were 0.25 and 0.30 ng mL−1 for MMP-9 and MMP-13, respectively. The developed immunosensor proved to be useful for the determination of both MMPs in saliva from healthy individuals and patients of periodontitis as well as in serum from patients diagnosed with rheumatoid arthritis (RA) and chronic obstructive pulmonary disease (COPD). The obtained results agreed with those provided by commercial ELISA kits, and the developed dual immunosensor proved to be competitive versus this methodology, requiring less than 2 h for the simultaneous determination of both biomarkers.
PB Springer Nature
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/124462
UL https://hdl.handle.net/20.500.14352/124462
LA eng
NO Ramos-López, C., García-Rodrigo, L., Sánchez-Tirado, E. et al. Nanocellulose-modified electrodes for simultaneous biosensing of microbiome-related oral diseases biomarkers. Microchim Acta 192, 141 (2025). https://doi.org/10.1007/s00604-025-07011-5
NO Ministerio de Ciencia e Innovacion
DS Docta Complutense
RD 18 dic 2025