RT Journal Article
T1 Metataxonomic Analysis and Fatty Acid Profiling of Feces from Children Undergoing Hematopoietic Stem Cell Transplantation
A1 Alba Rubio, Claudio
A1 Palomino, Laura
A1 Vergara, Beatriz
A1 Rodríguez-Belvis, Marta Velasco
A1 Aragón, Alberto
A1 Zaghlul, Marianna A. Di Campli
A1 Jurado Escobar, Rubén
A1 Martín-Fernández, Carmen
A1 Vázquez-Gómez, Julio A.
A1 González-Vicent, Marta
A1 Molina-Angulo, Blanca
A1 Sánchez-Llorente, Paula
A1 García-Hernández, Paloma
A1 Rodríguez Gómez, Juan Miguel
A1 Muñoz-Codoceo, Rosa A.
A1 Herranz Sorribes, Carmen
AB Allogeneic hematopoietic stem cell transplantation (HSCT) is a medical procedure to treat hematologic malignancies and restore bone marrow function. However, this approach may lead to graft-versus-host disease (GvHD), a major cause of mortality and morbidity after allogeneic HSCT. Some studies have suggested the involvement of gut microbiota in the development and prognosis of GvHD. In this context, the main objective of this study was to compare the fecal microbiome composition and short-chain profile of pediatric patients who underwent successful HSCT, developed GvHD or died. The bacterial composition was analyzed using 16S rRNA gene sequencing, while short-chain fatty acids (SCFAs) were quantified by gas chromatography. Fecal samples at engraftment were mainly characterized by a loss of bacterial diversity, a depletion of sequences belonging to the genus Blautia and significantly lower concentrations of fecal butyrate and acetate compared with those obtained before HSCT and 100 days after HSCT. Our findings confirm that children experiencing GvHD after HSCT have distinct gut microbiota and SCFA profiles, which might contribute to developing new microbiota-targeted strategies for GvHD prevention during HSCT procedures
PB MDPI
SN 1661-6596
YR 2026
FD 2026
LK https://hdl.handle.net/20.500.14352/134354
UL https://hdl.handle.net/20.500.14352/134354
LA eng
NO Alba, C., Palomino, L., Vergara, B., Rodríguez-Belvis, M. V., Aragón, A., Zaghlul, M. A. D. C., Jurado, R., Martín-Fernández, C., Vázquez-Gómez, J. A., González-Vicent, M., Molina-Angulo, B., Sánchez-Llorente, P., García-Hernández, P., Rodríguez, J. M., Muñoz-Codoceo, R. A., & Herranz, C. (2026). Metataxonomic Analysis and Fatty Acid Profiling of Feces from Children Undergoing Hematopoietic Stem Cell Transplantation. International journal of molecular sciences, 27(5), 2331. https://doi.org/10.3390/ijms27052331
NO Author ContributionsConceptualization, L.P., J.M.R. and R.A.M.-C.; methodology, L.P., B.V., M.A.D.C.Z., M.V.R.-B., C.M.-F., J.A.V.-G., M.G.-V., B.M.-A., P.S.-L., P.G.-H.; software, C.A.; validation, C.A., L.P., B.V., J.M.R., R.A.M.-C. and C.H.; formal analysis, C.A., L.P., B.V., J.M.R. and C.H.; investigation, C.A., A.A., R.J., C.H.; resources, L.P., J.M.R. and R.A.M.-C.; data curation, C.A. and C.H.; writing—original draft preparation, C.A., J.M.R., and C.H.; writing—review and editing, C.A., L.P., B.V., J.M.R., R.A.M.-C. and C.H.; visualization, C.A.; supervision, L.P., B.V., J.M.R. and R.A.M.-C.; project administration, L.P., J.M.R. and R.A.M.-C.; funding acquisition, L.P., J.M.R. and R.A.M.-C. All authors have read and agreed to the published version of the manuscript
NO Mutua Madrileña
NO Comunidad de Madrid
DS Docta Complutense
RD 5 abr 2026