RT Journal Article
T1 Comparative gene expression analysis of Porphyromonas gingivalis ATCC 33277 in planktonic and biofilms states
A1 Romero-Lastra, Patricia
A1 Sánchez Beltrán, María Del Carmen
A1 Ribeiro-Vidal, Honorato
A1 Llama Palacios, María Arantxazu
A1 Figuero Ruiz, Elena
A1 Herrera González, David
A1 Sanz Alonso, Mariano
AB Background and objectivePorphyromonas gingivalis is a keystone pathogen in the onset and progression of periodontitis. Its pathogenicity has been related to its presence and survival within the subgingival biofilm. The aim of the present study was to compare the genome-wide transcription activities of P. gingivalis in biofilm and in planktonic growth, using microarray technology.Material and methods P. gingivalis ATCC 33277 was incubated in multi-well culture plates at 37˚C for 96 hours under anaerobic conditions using an in vitro static model to develop both the planktonic and biofilm states (the latter over sterile ceramic calcium hydroxyapatite discs). The biofilm development was monitored by Confocal Laser Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM). After incubation, the bacterial cells were harvested and total RNA was extracted and purified. Three biological replicates for each cell state were independently hybridized for transcriptomic comparisons. A linear model was used for determining differentially expressed genes and reverse transcription quantitative polymerase chain reaction (RT-qPCR) was used to confirm differential expression. The filtering criteria of   ±2 change in gene expression and significance p-values of <0.05 were selected.ResultsA total of 92 out of 1,909 genes (4.8%) were differentially expressed by P. gingivalis growing in biofilm compared to planktonic. The 54 up-regulated genes in biofilm growth were mainly related to cell envelope, transport, and binding or outer membranes proteins. Thirty-eight showed decreased expression, mainly genes related to transposases or oxidative stress.ConclusionThe adaptive response of P. gingivalis in biofilm growth demonstrated a differential gene expression.
PB Public Library of Science
SN 1932-6203
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/94547
UL https://hdl.handle.net/20.500.14352/94547
LA eng
NO Romero-Lastra P, Sánchez MC, Ribeiro-Vidal H, Llama-Palacios A, Figuero E, Herrera D, Sanz M. Comparative gene expression analysis of Porphyromonas gingivalis ATCC 33277 in planktonic and biofilms states. PLoS One. 2017 Apr 3;12(4):e0174669.
DS Docta Complutense
RD 28 sept 2024