RT Journal Article
T1 Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley
A1 Bárány, Ivett
A1 Berenguer, Eduardo
A1 Solís González, María Teresa
A1 Pérez Pérez, Yolanda
A1 Santamaría, Estrella
A1 Crespo, José Luis
A1 Risueño, María
A1 Díaz, Isabel
A1 Testillano, Pilar
AB Microspores are reprogrammed towards embryogenesis by stress. Many microspores die after this stress, limiting the efficiency of microspore embryogenesis. Autophagy is a degradation pathway that plays critical roles in stress response and cell death. In animals, cathepsins have an integral role in autophagy by degrading autophagic material; less is known in plants. Plant cathepsins are papain-like C1A cysteine proteases involved in many physiological processes, including programmed cell death. We have analysed the involvement of autophagy in cell death, in relation to cathepsin activation, during stress-induced microspore embryogenesis in Hordeum vulgare. After stress, reactive oxygen species (ROS) and cell death increased and autophagy was activated, including HvATG5 and HvATG6 up-regulation and increase of ATG5, ATG8, and autophagosomes. Concomitantly, cathepsin L/F-, B-, and H-like activities were induced, cathepsin-like genes HvPap-1 and HvPap-6 were up-regulated, and HvPap-1, HvPap-6, and HvPap-19 proteins increased and localized in the cytoplasm, resembling autophagy structures. Inhibitors of autophagy and cysteine proteases reduced cell death and promoted embryogenesis. The findings reveal a role for autophagy in stress-induced cell death during microspore embryogenesis, and the participation of cathepsins. Similar patterns of activation, expression, and localization suggest a possible connection between cathepsins and autophagy. The results open up new possibilities to enhance microspore embryogenesis efficiency with autophagy and/or cysteine protease modulators.
PB Oxford University Press
SN 0022-0957
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/96741
UL https://hdl.handle.net/20.500.14352/96741
LA eng
NO Bárány I, Berenguer E, Solís MT, Pérez-Pérez Y, Santamaría ME, Crespo JL, Risueño MC, Díaz I, Testillano PS. Autophagy is activated and involved in cell death with participation of cathepsins during stress-induced microspore embryogenesis in barley. J Exp Bot. 2018 Mar 14;69(6):1387-1402. doi: 10.1093/jxb/erx455. PMID: 29309624; PMCID: PMC6019037.
NO This work is supported by projects AGL2014-52028-R and AGL2017-82447-R funded by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (ERDF/FEDER). YPP is the recipient of a grant (PEJ15/BIO/AI-01S8) funded by Comunidad de Madrid and European Commission through ERDF/FEDER.
NO Comunidad de Madrid
NO Ministerio de Economía y Competitividad (España)
NO European Commission
DS Docta Complutense
RD 6 oct 2024