RT Journal Article
T1 Specific stress responses to cadmium, arsenic and mercury appear in the metallophyte Silene vulgaris when grown hydroponically
A1 Sobrino Plata, Juan
A1 Herrero, Joaquín
A1 Carrasco-Gil, Sandra
A1 Pérez-Sanz, Araceli
A1 Lobo, Carmen
A1 Escobar, Carolina
A1 Millán, Rocío
A1 Hernández, Luis
AB The tolerance of the metallophyte Silene vulgaris, a plant suitable for the phytostabilisation of metal(loid)-contaminated soils, to arsenic (As), mercury (Hg) and cadmium (Cd) was evaluated in a semi-hydroponic culture system under controlled environmental conditions. The appearance of oxidative stress, alteration of photochemical processes and modification of biothiol content were studied as physiological parameters of metal(loid) stress in plants treated with 0, 6 and 30 μM (As, Hg or Cd) for 7 days. In spite of the metal(loid) excluder behaviour of S. vulgaris, Cd was translocated to the aerial part of the plant at a higher rate than Hg or As. The major toxic effects were observed in roots, where lipid peroxidation was increased in a dose-dependent manner. Redox enzymes such as glutathione reductase (GR) were severely inhibited by Hg, whereas GR was overexpressed. The accumulation of Cd produced a remarkable production of phytochelatins (PCs) in roots, whereas Hg and As led to modest PCs synthesis. There was a severe loss of chlorophyll content in Cd-treated plants, accompanied with a significant decrease in photosystem II efficiency (ΦPSII) and photochemical quenching (qP). Similar negative effects were observed in Hg- and As-exposed plants, but to a lesser degree. The exposure to the highest dose of each toxic element (30 μM) caused depletion of the light harvesting complex b1 protein. In conclusion, specific stress signatures to each metal(loid) were observed, with As being the least toxic element, suggesting that different mechanisms of tolerance were exerted. These results could be applied in future experiments to select tolerant ecotypes to optimize the phytostabilisation of metal(loid) multipolluted soils.
PB Royal Society of Chemistry
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/99645
UL https://hdl.handle.net/20.500.14352/99645
LA eng
NO Sobrino-Plata, Juan, et al. «Specific Stress Responses to Cadmium, Arsenic and Mercury Appear in the Metallophyte Silene Vulgaris When Grown Hydroponically». RSC Advances, vol. 3, n.o 14, 2013, p. 4736. https://doi.org/10.1039/c3ra40357b.
DS Docta Complutense
RD 7 abr 2025