RT Journal Article
T1 Transcriptome analysis of rainbow trout in response to non-virion (NV) protein of Viral Haemorrhagic Septicaemia virus (VHSV)
A1 Chinchilla Rodríguez, Blanca
A1 Encinas, Paloma
A1 Estepa, Amparo
A1 Coll Morales, Julio
A1 Gómez Casado, Eduardo
AB The non-virion (NV) protein of viral haemorrhagic septicaemia virus (VHSV), an economically important fish novirhabdovirus, has been implicated in the interference of some host innate mechanisms (i.e. apoptosis) in vitro. This work aimed to characterise the immune-related transcriptome changes in rainbow trout induced by NV protein that have not yet been established in vivo. For that purpose, immune-targeted microarrays were used to analyse the transcriptomes from head kidney and spleen of rainbow trout (Oncorhynchus mykiss) after injection of recombinant NV (rNV). Results showed the extensive downregulation (and in some cases upregulation) of many innate and adaptive immune response genes not related previously to VHSV infection. The newly identified genes belonged to VHSV-induced genes (vigs), tumour necrosis factors, Toll-like receptors, antigen processing and presentation, immune co-stimulatory molecules, interleukins, macrophage chemotaxis, transcription factors, etc. Classification of differentially downregulated genes into rainbow trout immune pathways identified stat1 and jun/atf1 transcription factor genes as the most representative of the multipath gene targets of rNV. Altogether, these results contribute to define the role and effects of NV in trout by orchestrating an immunosuppression of the innate immune responses for favouring viral replication upon VHSV infection. Finally, these transcriptome results open up the possibility to find out new strategies against VHSV and better understand the interrelationships between some immune pathways in trout.
PB SpringerLink
SN 0175-7598
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/97345
UL https://hdl.handle.net/20.500.14352/97345
LA eng
NO Chinchilla, B., Encinas, P., Estepa, A., Coll, J. M., & Gomez-Casado, E. (2015). Transcriptome analysis of rainbow trout in response to non-virion (NV) protein of viral haemorrhagic septicaemia virus (VHSV). Applied microbiology and biotechnology, 99(4), 1827–1843. https://doi.org/10.1007/s00253-014-6366-3
NO Blanca Chinchilla is a research fellow FPI (2012) of the Ministerio of the Ministry of Economy and Competitiveness of Spain.
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 17 abr 2025