RT Journal Article
T1 Toll-like receptor 4 deficient mice do not develop remifentanil-induced mechanical hyperalgesia
A1 Aguado Domínguez, Delia
A1 Bustamante Domínguez, Rocío
A1 Álvarez Gómez De Segura, Ignacio
AB Background: Drugs with antagonistic actions on the Toll-like receptor 4 (Tlr4), such as naloxone at ultra low doses, have been used to inhibit opioid-induced hyperalgesia in rodents suggesting the involvement of this receptor and pathway on opioid-induced hyperalgesia.Objective: The aim of this study was to determine whether mice without the Tlr4 gene (Tlr4) would not develop remifentanil-induced hyperalgesia.Design: An experimental randomised animal study.Setting: Experimental Unit, Complutense University of Madrid, Madrid, Spain.Animals: Twelve adult female wild-type mice and 12 adult Tlr4 mice.Interventions: Under sevoflurane anaesthesia, a 1-h, constant rate subcutaneous infusion of remifentanil (4 μg kg min) or 0.9% saline.Main outcome measures: Mechanical nociceptive thresholds were evaluated using a von Frey hair test before (baseline) and on days 5, 6 and 7 after treatment. Hyperalgesia was considered to be a decrease in the mechanical nociceptive threshold. Changes in mechanical nociceptive thresholds in the different groups were compared with one-sided paired t tests.Results: Baseline mechanical nociceptive thresholds were similar in all groups (2.2 ± 0.1 g). Remifentanil produced a 24% decrease in mechanical nociceptive thresholds in the wild-type mice (1.7 ± 0.0 g, averaged over 3 days, P = 0.00021), whereas the nociceptive thresholds were not changed in Tlr4 mice (2.2 ± 0.1 g, P = 0.857) or in mice receiving 0.9% saline (Tlr4, 2.2 ± 0.1 g, P = 0.807; wild-type, 2.2 ± 0.1 g, P = 0.962).Conclusion: Tlr4 receptor involvement is suggested in the development of remifentanil-induced hyperalgesia in mice.Trial registration: CEA-UCM 107/2012.
PB Lippincott Williams and Wilkins
SN 0265-0215
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/101707
UL https://hdl.handle.net/20.500.14352/101707
LA eng
NO Aguado, Delia; Bustamante, Rocío; Gómez de Segura, Ignacio A.. Toll-like receptor 4 deficient mice do not develop remifentanil-induced mechanical hyperalgesia: An experimental randomised animal study. European Journal of Anaesthesiology 35(7):p 505-510, July 2018. | DOI: 10.1097/EJA.0000000000000803
NO Instituto de Salud Carlos III
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 6 abr 2025