RT Journal Article
T1 Solid lipid nanoparticles for ocular delivery of isoniazid: evaluation, proof of concept and in vivo safety & kinetics
A1 Singh, Mandeep
A1 Guzmán Aránguez, Ana Isabel
A1 Hussain, Afzal
A1 Srinivas, Cheerneni S.
A1 Kaur, Indu P.
AB Aim: Evaluation of solid lipid nanoparticles (SLNs) for ocular delivery of isoniazid (INH). Materials & methods: INH-SLNs were characterized for morphological, thermal, crystalline and nuclear magnetic resonance properties. In vitro release and ex vivo corneal permeability of INH-SLNs was also evaluated. Proof-of-concept uptake studies were performed in corneal and conjunctival cell lines and in vivo in rat eye using fluorescein-labeled SLNs. Antimycobacterial activity of INH-SLNs was confirmed. In vivo aqueous humor pharmacokinetics, toxicity and tolerance was performed in rabbit/rat eye. Results: INH-SLNs showed extended release (48 h), enhanced corneal permeability (1.6-times), five-times lower MIC, significant in vitro and in vivo uptake of fluorescein-labeled SLNs, 4.2-times ocular bioavailability (area under the curve) and in vivo acute and repeat dose safety. Conclusion: INH-SLNs are an effective ocular delivery system.
PB Future Medicine
SN 1743-5889
YR 2019
FD 2019-01-29
LK https://hdl.handle.net/20.500.14352/13263
UL https://hdl.handle.net/20.500.14352/13263
LA eng
NO Department of Biotechnology (DBT), New Delhi, India
DS Docta Complutense
RD 10 abr 2025