RT Journal Article
T1 Polymerase chain reaction (PCR) identification of Penicillium brevicompactum, a grape contaminant and mycophenolic acid producer
A1 Patiño Álvarez, Aurora Belén
A1 Medina, Á.
A1 Domenech Lucas, Miriam
A1 González Jaén, María Teresa
A1 Jiménez, M.
A1 Vázquez, C.
AB Penicillium brevicompactum is a ubiquitous fungal species that contaminates diverse substrates and commodities and produces an array of metabolites toxic to human and animals. The present work has obtained evidence, by liquid chromatography (LC)-ion-trap mass spectrometry, of the ability of P. brevicompactum strains isolated from grapes to produce mycophenolic acid, a potent immunosuppressor. In order to facilitate early diagnosis of this species on commodities for human and animal consumption, a rapid, sensitive and specific polymerase chain reaction (PCR) assay for P. brevicompactum was developed. The specific primers were designed based on the ITS1-5.8S-ITS2ITS (Internal Transcribed Spacers of rRNA genes) multicopy region. This method provides a useful aid to detect the presence of this fungal species in grapes and other commodities in order to prevent the toxins produced entering the food chain.
SN 0265-203X
YR 2007
FD 2007-02
LK https://hdl.handle.net/20.500.14352/98309
UL https://hdl.handle.net/20.500.14352/98309
LA eng
NO Ministerio de Educación y Ciencia
NO Gobierno de Valencia
DS Docta Complutense
RD 22 jul 2024