RT Journal Article
T1 Position-dependent expression of GADD45 alpha in rat brain tumours
A1 Bru Espino, Antonio Leonardo
A1 Fresno, Carlos del
A1 Soares Schanoski, Alessandra
A1 Albertos, Sonia
A1 Brú, Isabel
A1 Porres, Amelia
A1 Rollán Landeras, Eduardo Ricardo
A1 Dopazo, Ana
A1 Casero Díaz-Cano, David
A1 Gomez Piña, Vanesa
A1 Garcia, Lourdes
A1 Arnalich, Francisco
A1 Alvarez, Rebeca
A1 Rodriguez-Rojas, Alexandro
A1 Fuentes-Prior, Pablo
A1 López-Collazo, Eduardo
AB Although the complex and multifactorial process of tumour growth has been extensively studied for decades, our understanding of the fundamental relationship between tumour growth dynamics and genetic expression profile remains incomplete. Recent studies of tumour dynamics indicate that gene expression in solid tumours would depend on the distance from the centre of the tumour. Since tumour proliferative activity is mainly localised to its external zone, and taking into account that generation and expansion of genetic mutations depend on the number of cell divisions, important differences in gene expression between central and peripheral sections of the same tumour are to be expected. Here, we have studied variations in the genetic expression profile between peripheral and internal samples of the same brain tumour. We have carried out microarray analysis of mRNA expression, and found a differential profile of genetic expression between the two cell subsets. In particular, one major nuclear protein that regulates cell responses to DNA-damaging and stress signals, GADD45 alpha, was expressed at much lower levels in the peripheral zone, as compared to tumour core samples. These differences in GADD45 alpha mRNA transcription levels have been confirmed by quantitative analysis via real time PCR, and protein levels of GADD45 alpha also exhibit the same pattern of differential expression. Our findings suggest that GADD45 alpha might play a major role in the regulation of brain tumour invasive potential.
PB HUMANA PRESS INC
SN 1357-0560
YR 2007
FD 2007-06-19
LK https://hdl.handle.net/20.500.14352/49838
UL https://hdl.handle.net/20.500.14352/49838
LA eng
NO Bru Espino, A. L., Frescno, C., Soares Schanoski, A. et al. «Position-Dependent Expression of GADD45α in Rat Brain Tumours». Medical Oncology, vol. 24, n.o 4, diciembre de 2007, pp. 436-44. DOI.org (Crossref), https://doi.org/10.1007/s12032-007-0025-9.
NO Fundación Médica Mutua Madrileña Automovilística
NO Universidad Complutense
NO European Contract
DS Docta Complutense
RD 5 abr 2025