%0 Journal Article
%A Luque Uría, Álvaro
%A Peltomaa, Riikka
%A Navarro Duro, Marina
%A Fikacek, Sabrina
%A Head, Trajen
%A Deo, Sapna K.
%A Daunert, Sylvia
%A Benito Peña, María Elena
%A Moreno Bondi, María Cruz
%T Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
%D 2022
%@ 2629-2742
%U https://hdl.handle.net/20.500.14352/71361
%X The development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.
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