RT Journal Article
T1 Comparative Study of the Performance of Two Different Luciferases for the Analysis of Fumonisin B1 in Wheat Samples
A1 Luque Uría, Álvaro
A1 Peltomaa, Riikka
A1 Navarro Duro, Marina
A1 Fikacek, Sabrina
A1 Head, Trajen
A1 Deo, Sapna K.
A1 Daunert, Sylvia
A1 Benito Peña, María Elena
A1 Moreno Bondi, María Cruz
AB The development of two different immunoassays for the determination of fumonisin B1 in wheat samples is reported. A previously described mimopeptide for fumonisin B1 (FB1) was used to produce fusion proteins in combination with two different luciferases: Gaussia luciferase (GLuc) and NanoLuc luciferase (NLuc). The production, expression and the development of two immunoassays based on these fusion proteins (A2- GLuc and A2-NLuc) is detailed. The assay showing the best performance, A2-NLuc, with a limit of detection of 0.61 ngmL 1 and a dynamic range from 1.9 to 95 ngmL 1 , was employed for the analysis of spiked wheat samples, a reference matrix material, as well as naturally contaminated wheat samples. The recoveries obtained in the spiked samples were acceptable, between 81.5 and 109%, with relative standard deviations lower than 14%. The analysis of naturally contaminated wheat was validated by a liquid chromatography coupled to tandem mass detection method.
PB Wiley-VCH
SN 2629-2742
YR 2022
FD 2022-02-17
LK https://hdl.handle.net/20.500.14352/71361
UL https://hdl.handle.net/20.500.14352/71361
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2022)
NO Ministerio de Ciencia e Innovación (MICINN)
DS Docta Complutense
RD 20 jul 2024