RT Journal Article
T1 The chemoattraction of lymphocytes by rheumatoid arthritis - synovial fluid is not dependent on the chemokine receptor CCR5
A1 Santiago, Begoña
A1 Galindo Izquierdo, María
A1 Rivero, Miguel
A1 Brehmer, M.
A1 Mateo, I.
A1 Pablos Álvarez, José Luis
AB Objective: The objective was to study the potential role of the chemokine receptor CCR5 in the chemoattraction of lymphocytes by rheumatoid arthritis synovial fluid (RA-SF).Methods: The expression of the CCR5 receptor was studied by flow cytometry. Chemotaxis of peripheral blood lymphocytes in response to RA-SF was analyzed on transmigration chambers. Chemotaxis of immortalized lymphocytes from individuals homozygous for the Delta32 deletion of the CCR5 gene (CCR5-/-) was analyzed. The effect of a neutralizing anti-CCR5 antibody on the migration of CCR5+/+ cells was also studied.Results: We confirmed an increase in the proportion of CCR5-expressing lymphocytes in RA-SF and a preferential migration of CCR5+ lymphocytes toward RA-SF in vitro. CCR5-/- lymphocytes showed decreased chemotactic responses to the chemokine MIP-1beta but not to RA-SF. The chemotactic responses of CCR5+/+ lymphocytes to RA-SF were not modified by anti-CCR5 neutralizing antibody.Conclusions: We confirm a preferential accumulation of CCR5-expressing lymphocytes into RA-SF. However, the chemotactic responses of lymphocytes to RA-SF were not dependent on a functional CCR5 receptor, suggesting that CCR5 is a marker of a lymphocyte subset rather than a specific mediator of chemotactic responses to chemokines in RA-SF.
PB Springer
SN 0172-8172
YR 2002
FD 2002-07-01
LK https://hdl.handle.net/20.500.14352/114228
UL https://hdl.handle.net/20.500.14352/114228
LA eng
NO Santiago B, Galindo M, Rivero M, Brehmer MT, Mateo I, Pablos JL. The chemoattraction of lymphocytes by rheumatoid arthritis - synovial fluid is not dependent on the chemokine receptor CCR5. Rheumatol Int. 2002 Jul;22(3):107-11.
NO Ministerio de Educacion y Cultura (España)
NO Ministerio de Sanidad y Consumo (España)
DS Docta Complutense
RD 6 abr 2025