RT Journal Article
T1 Recombinant antibodies and their use for food immunoanalysis
A1 Peltomaa, Riikka Johanna
A1 Barderas Manchado, Rodrigo
A1 Benito Peña, María Elena
A1 Moreno Bondi, María Cruz
AB Antibodies are widely employed as biorecognition elements for the detection of a plethora of compounds including food and environmental contaminants, biomarkers, or illicit drugs. They are also applied in therapeutics for the treatment of several disorders. Recent recommendations from the EU on animal protection and the replacement of animal-derived antibodies by non-animal-derived ones have raised a great controversy in the scientific community. The application of recombinant antibodies is expected to achieve a high growth rate in the years to come thanks to their versatility and beneficial characteristics in comparison to monoclonal and polyclonal antibodies, such as stability in harsh conditions, small size, relatively low production costs, and batch-to-batch reproducibility. This review describes the characteristics, advantages, and disadvantages of recombinant antibodies including antigen-binding fragments (Fab), single-chain fragment variable (scFv), and single-domain antibodies (VHH) and their application in food analysis with especial emphasis on the analysis of biotoxins, antibiotics, pesticides, and foodborne pathogens. Although the wide application of recombinant antibodies has been hampered by a number of challenges, this review demonstrates their potential for the sensitive, selective, and rapid detection of food contaminants.
PB Springer
SN 1618-2642
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/92749
UL https://hdl.handle.net/20.500.14352/92749
LA eng
NO European Commission
DS Docta Complutense
RD 10 abr 2025