RT Journal Article
T1 Immune Analysis Using Vitreous Optical Coherence Tomography Imaging in Rats with Steroid-Induced Glaucoma
A1 Aragón Navas, Alba
A1 Bravo Osuna, Irene
A1 Herrero Vanrell, María Del Rocío
A1 Rodrigo, María Jesús
A1 Subías, Manuel
A1 Montolío, Alberto
A1 Martínez-Rincón, Teresa
A1 Pablo, Luis E
A1 Cegoñino, Jose
A1 García-Martín, Elena
A1 Pérez del Palomar, Amaya
AB Glaucoma is a multifactorial pathology involving the immune system. The subclinical immune response plays a homeostatic role in healthy situations, but in pathological situations, it produces imbalances. Optical coherence tomography detects immune cells in the vitreous as hyperreflective opacities and these are subsequently characterised by computational analysis. This study monitors the changes in immunity in the vitreous in two steroid-induced glaucoma (SIG) animal models created with drug delivery systems (microspheres loaded with dexamethasone and dexamethasone/fibronectin), comparing both sexes and healthy controls over six months. SIG eyes tended to present greater intensity and a higher number of vitreous opacities (p < 0.05), with dynamic fluctuations in the percentage of isolated cells (10 μm2), non-activated cells (10–50 μm2), activated cells (50–250 μm2) and cell complexes (>250 μm2). Both SIG models presented an anti-inflammatory profile, with non-activated cells being the largest population in this study. However, smaller opacities (isolated cells) seemed to be the first responder to noxa since they were the most rounded (recruitment), coinciding with peak intraocular pressure increase, and showed the highest mean Intensity (intracellular machinery), even in the contralateral eye, and a major change in orientation (motility). Studying the features of hyperreflective opacities in the vitreous using OCT could be a useful biomarker of glaucoma.
PB MDPI
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/118077
UL https://hdl.handle.net/20.500.14352/118077
LA eng
NO Rodrigo MJ, Subías M, Montolío A, Martínez-Rincón T, Aragón-Navas A, Bravo-Osuna I, Pablo LE, Cegoñino J, Herrero-Vanrell R, Garcia-Martin E, Pérez Del Palomar A. Immune Analysis Using Vitreous Optical Coherence Tomography Imaging in Rats with Steroid-Induced Glaucoma. Biomedicines 2024;12:633. https://doi.org/10.3390/biomedicines12030633.
NO European Commission
NO European Social Fund Plus
NO European Regional Development Fund
NO Instituto de Salud Carlos III (España)
NO Gobierno de Aragón (España)
DS Docta Complutense
RD 5 abr 2025