RT Journal Article
T1 Influence of the helical backbone in the behavior of a simple model for dimeric coiled‐coil proteins
A1 Prieto Frías, Lidia
A1 Rey Gayo, Antonio
AB Using computer simulations as a tool for thought experiments, we investigate the influence of the helical backbone geometry in the association process and the final structures of a simple model which mimics parallel, two‐stranded coiled‐coil proteins. We define three types of helices: two of them have straight helical axes and 3.5 or 3.6 residues per helical turn; the third type presents a coiled helical axis, according to the canonical scheme defined by Crick. By using a Monte Carlo simulation algorithm, we observe that the three models exhibit different transition temperatures for the formation of the dimeric structure from two independent peptides, and a different behavior concerning the appearance of out‐of‐register structures. The energy minimized dimer structures present strong deviations from the correct association for straight helices with 3.6 residues/turn, especially for long peptides, deviations which are absent for the other two types when only the burial of hydrophobic residues is considered. A careful analysis of the energies for the out‐of‐register configurations and the contact maps reveals also differences between dimers resulting from the model with Crick parameterization and with 3.5 residues/turn. The results presented in this paper may be relevant for the design of simple models which use rigid α‐helices built from predicted elements of secondary structure.
PB Wiley-VCH Verlag
SN 1022-1344
YR 2003
FD 2003
LK https://hdl.handle.net/20.500.14352/91112
UL https://hdl.handle.net/20.500.14352/91112
LA eng
NO Prieto, L. and Rey, A. (2003). Influence of the helical backbone in the behavior of a simple model for dimeric coiled-coil proteins. Macromol. Theory Simul., 12: 669-678.
NO Ministerio de Ciencia y Tecnología (España)
NO Ministerio de Educación, Cultura y Deportes (España)
DS Docta Complutense
RD 16 abr 2025