RT Journal Article
T1 Multi-omics framework to reveal the molecular determinants of fermentation performance in wine yeast populations
A1 De Celis Rodríguez, Miguel
A1 Ruiz Ruiz, Javier
A1 Benítez Domínguez, Belén
A1 Vicente Sánchez, Javier
A1 Tomasi, Sandra
A1 Izquierdo Gea, Sergio
A1 Rozes, Nicolás
A1 Ruiz de Villa, Candela
A1 Gombau, Jordi
A1 Zamora, Fernando
A1 Barroso del Jesús, Alicia
A1 Terrón Camero, Laura C.
A1 Andrés León, Eduardo
A1 Santos de la Sen, Antonio
A1 Belda Aguilar, Ignacio
AB BackgroundConnecting the composition and function of industrial microbiomes is a major aspiration in microbial biotechnology. Here, we address this question in wine fermentation, a model system where the diversity and functioning of fermenting yeast species are determinant of the flavor and quality of the resulting wines.ResultsFirst, we surveyed yeast communities associated with grape musts collected across wine appellations, revealing the importance of environmental (i.e., biogeography) and anthropic factors (i.e., farming system) in shaping community composition and structure. Then, we assayed the fermenting yeast communities in synthetic grape must under common winemaking conditions. The dominating yeast species defines the fermentation performance and metabolite profile of the resulting wines, and it is determined by the initial fungal community composition rather than the imposed fermentation conditions. Yeast dominance also had a more pronounced impact on wine meta-transcriptome than fermentation conditions. We unveiled yeast-specific transcriptomic profiles, leveraging different molecular functioning strategies in wine fermentation environments. We further studied the orthologs responsible for metabolite production, revealing modules associated with the dominance of specific yeast species. This emphasizes the unique contributions of yeast species to wine flavor, here summarized in an array of orthologs that defines the individual contribution of yeast species to wine ecosystem functioning.ConclusionsOur study bridges the gap between yeast community composition and wine metabolite production, providing insights to harness diverse yeast functionalities with the final aim to producing tailored high-quality wines.
PB BioMed Central
YR 2024
FD 2024-10
LK https://hdl.handle.net/20.500.14352/109968
UL https://hdl.handle.net/20.500.14352/109968
LA eng
NO de Celis, M., Ruiz, J., Benitez-Dominguez, B. et al. Multi-omics framework to reveal the molecular determinants of fermentation performance in wine yeast populations. Microbiome 12, 203 (2024). https://doi.org/10.1186/s40168-024-01930-w
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Universidad Complutense de Madrid
NO Comunidad de Madrid
NO Junta de Andalucía
DS Docta Complutense
RD 16 abr 2025