RT Journal Article
T1 Interferon-gamma responses in sheep exposed to virulent and attenuated Brucella melitensis strains
A1 Pérez Sancho, Marta
A1 Durán-Ferrer, Manuel
A1 García-Seco Romero, María Teresa
A1 Macías, Paula
A1 García Benzaquén, Nerea
A1 Martínez Alares, Irene
A1 Ruiz, Elena
A1 Legaz, Emilio
A1 Díez Guerrier, Alberto Antoine
A1 González Domínguez, Sergio
A1 Domínguez Rodríguez, Lucas José
A1 Álvarez Sánchez, Julio
AB Antibody detection is the basis of large-scale sheep brucellosis diagnosis because of its sensitivity and specificity. In contrast, information on the cellular mediated immune (CMI) response triggered after Brucella melitensis infection, a cornerstone in the protection against this pathogen, is more limited, particularly regarding the effect of the virulence of the infecting strain in the induced CMI reaction. Here, the interferon-gamma (IFN-gamma) profiles evoked after exposure by different routes to virulent (H38) and attenuated (Rev.1) B. melitensis strains in 14 pregnant sheep and 87 ewe lambs, respectively, were characterized accounting for different host-related factors, and compared with their serological response and with the basal IFN-gamma responses observed in 155 animals non exposed to Brucella. No significant differences in the IFN-gamma response of Rev.1 vaccinated animals depending on the inoculation route was observed, in contrast with their serological results. Response in H38- challenged followed a similar trend although peaked later, and an effect of the abortion on the IFN-gamma response was detected. This information could help to understand the interaction bacteria–host that leads to its intracellular survival and could be useful for the design of new diagnostic approaches.
PB Elsevier
SN 0165-2427
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/93254
UL https://hdl.handle.net/20.500.14352/93254
LA eng
NO Pérez-Sancho, Marta, et al. «Interferon-Gamma Responses in Sheep Exposed to Virulent and Attenuated Brucella Melitensis Strains». Veterinary Immunology and Immunopathology, vol. 160, n.o 1-2, julio de 2014, pp. 123-28. https://doi.org/10.1016/j.vetimm.2014.03.014.
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
NO European Commission 
DS Docta Complutense
RD 6 jul 2025