RT Journal Article
T1 Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation
A1 Bouthelier, Antonio
A1 Fernández- Arroyo, Lucía
A1 Mesa Ciller, Claudia
A1 Cibrian, Danay
A1 Martín Cofreces, Noa Beatriz
A1 Castillo González, Raquel
A1 Calero Calero, Macarena
A1 Herráez Aguilar, Diego
A1 Guajardo Grence, Andrea
A1 Pacheco, Ana María
A1 Marcos Jiménez, Ana
A1 Quiroga, Borja
A1 Morado, Marta
A1 Morado, Marta
A1 Monroy Muñoz, Francisco
A1 Muñoz Calleja, Cecilia
A1 Sánchez Madrid, Francisco
A1 Urrutia, Andrés A
A1 Aragonés, Julián
AB Inhibition of the heterodimeric amino acid carrier SLC7A5/SLC3A2 (LAT1/CD98) has been widely studied in tumor biology but its role in physiological conditions remains largely unknown. Here we show that the SLC7A5/SLC3A2 heterodimer is constitutively present at different stages of erythroid differentiation but absent in mature erythrocytes. Administration of erythropoietin (EPO) further induces SLC7A5/SLC3A2 expression in circulating reticulocytes, as it also occurs in anemic conditions. Although Slc7a5 gene inactivation in the erythrocyte lineage does not compromise the total number of circulating red blood cells (RBCs), their size and hemoglobin content are significantly reduced accompanied by a diminished erythroblast mTORC1 activity. Furthermore circulating Slc7a5-deficient reticulocytes are characterized by lower transferrin receptor (CD71) expression as well as mitochondrial activity, suggesting a premature transition to mature RBCs. These data reveal that SLC7A5/SLC3A2 ensures adequate maturation of reticulocytes as well as the proper size and hemoglobin content of circulating RBCs.
PB Elsevier
SN 2589-0042
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/93189
UL https://hdl.handle.net/20.500.14352/93189
LA eng
NO Bouthelier, A.; Fernández-Arroyo, L.; Mesa-Ciller, C.; Cibrian, D.; Martín-Cófreces, N. B.; Castillo-González, R.; Calero, M.; Herráez-Aguilar, D.; Guajardo-Grence, A.; Pacheco, A. M.; Marcos-Jiménez, A.; Quiroga, B.; Morado, M.; Monroy, F.; Muñoz-Calleja, C.; Sánchez-Madrid, F.; Urrutia, A. A.; Aragonés, J. Erythroid SLC7A5/SLC3A2 amino acid carrier controls red blood cell size and maturation. iScience 2023, 26, 105739 DOI:10.1016/j.isci.2022.105739.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 17 abr 2025