RT Journal Article
T1 Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy
A1 Velloso Álvarez, Ana
A1 Jose Cunilleras, E.
A1 Dorrego Rodríguez, Abel
A1 Santiago Llorente, Isabel
A1 De la Cuesta Torrado, María
A1 Troya Portillo, Lucas
A1 Rivera Arroyo, Belén
A1 Vitale, Valentina
A1 Juan Ferré, Lucía De
A1 Cruz López, Fátima
AB Background: Real-time PCR is the diagnostic technique of choice for the diagnosis and control of equine herpesvirus-1 (EHV-1) in an outbreak setting. The presence of EHV-1 in nasal swabs (NS), whole blood, brain and spinal cord samples has been extensively described; however, there are no reports on the excretion of EHV-1 in urine, its DNA detection patterns, and the role of urine in viral spread during an outbreak.Objectives: To determine the presence of EHV-1 DNA in urine during natural infection and to compare the DNA detection patterns of EHV-1 in urine, buffy coat (BC) and NS.Study design: Descriptive study of natural infection.Methods:  Urine and whole blood/NS samples were collected at different time points during the hospitalisation of 21 horses involved in two EHV-1 myeloencephalopathy outbreaks in 2021 and 2023 in Spain. Quantitative real-time PCR was performed to compare the viral DNA load between BC-urine samples in 2021 and NS-urine samples in 2023. Sex, age, breed, presence of neurological signs, EHV-1 vaccination status and treatment data were recorded for all horses.Results: A total of 18 hospitalised horses during the 2021 and 2023 outbreaks were positive for EHV-1, and viral DNA was detected in urine samples from a total of 11 horses in both outbreaks. Compared with BC samples, DNA presence was detected in urine samples for longer duration and with slightly higher concentration; however, compared with NS, detection of EHV-1 in urine was similar in duration with lower DNA concentrations.Main limitations: Limited sample size, different sampling times and protocols (BC vs. NS) in two natural infection outbreak settings.Conclusions: EHV-1 was detected in the urine from naturally infected horses. Urine should be considered as complimentary to blood and NS in diagnosis of EHV-1 infection.
PB Wiley
SN 0425-1644
YR 2023
FD 2023-09-12
LK https://hdl.handle.net/20.500.14352/104378
UL https://hdl.handle.net/20.500.14352/104378
LA eng
NO Velloso-Alvarez A, Jose-Cunilleras E, Dorrego-Rodriguez A, Santiago-Llorente I, de las Cuesta-Torrado M, Troya-Portillo L, Rivera B, Vitale V, de Juan L and Cruz F*. Detection of equine herpesvirus-1 (EHV-1) in urine samples during outbreaks of equine herpesvirus myeloencephalopathy. Equine veterinary journal, 56(3):456-463. 2024. (A). ISSN: 0425-1644. Impact factor 2022: 2.200. Category: Veterinary Sciences, Quartile: 2, Position: 38 of 143. DOI: 10.1111/evj.14007
NO 2023 Acuerdos transformativos CRUE
DS Docta Complutense
RD 10 sept 2024