RT Journal Article T1 Incidence and Risk Factors of Surgical Site Infection in 376 Mastectomy Procedures in Female Dogs: A Retrospective Cohort Study A1 Fuertes Recuero, Manuel A1 Penelo Hidalgo, Silvia A1 Suarez-Redondo, María A1 Eceiza-Zubicaray, Alba A1 Arenillas Baquero, Mario A1 Valdivia Lara, Edgar Guillermo A1 García San José, Paula A1 Peña Fernández, Laura Luisa A1 Pérez Alenza, María De Los Dolores A1 Ortiz Díez, Gustavo AB The incidence of surgical site infection (SSI) following mastectomy in female dogs remains poorly characterized, despite its relevance to postoperative outcomes and patient welfare. This retrospective cohort study aimed to determine the incidence of SSI and identify associated clinical and surgical risk factors in dogs undergoing mastectomy procedures at a veterinary teaching hospital between 2013 and 2022. SSI diagnosis was based on the Centers for Disease Control (CDC) criteria and identified structured passive surveillance of medical records. Univariate logistic regression was carried out to screen for potential risk factors, followed by multivariate analysis of selected variables. Among 376 mastectomy procedures performed in 306 dogs, 33 SSIs were detected, resulting in an overall incidence of 8.8% (95% CI: 6.1-12.1). The majority were superficial infections (93.9%), with only two cases classified as deep SSI; no organ/space infections were recorded. Prolonged anaesthesia duration ( = 0.041) and intraoperative hypothermia ( = 0.026) were independently associated with increased SSI risk. These findings indicate that the incidence of SSI after canine mastectomy is comparable to that reported for other clean surgeries. Prolonged anaesthesia and hypothermia represent modifiable risk factors, highlighting the importance of perioperative temperature control and optimized anaesthetic management. No significant associations were observed with patient-related variables, mastectomy type, or wound soaker catheter use, supporting its safe inclusion in multimodal analgesia. PB MDPI YR 2025 FD 2025 LK https://hdl.handle.net/20.500.14352/122765 UL https://hdl.handle.net/20.500.14352/122765 LA eng NO Fuertes-Recuero, M.; Penelo, S.; Suarez-Redondo, M.; Eceiza-Zubicaray, A.; Arenillas, M.; Valdivia, G.; García San José, P.; Peña, L.; Pérez-Alenza, D.; Ortiz-Díez, G. Incidence and Risk Factors of Surgical Site Infection in 376 Mastectomy Procedures in Female Dogs: A Retrospective Cohort Study. Vet. Sci. 2025, 12, 553. https://doi.org/ 10.3390/vetsci12060553 NO Justificación de autores:Conceptualization, M.F.-R., S.P., M.S.-R., A.E.-Z., M.A., G.V., P.G.S.J., L.P., D.P.-A. and G.O.-D.; methodology, M.F.-R., S.P., M.S.-R., A.E.-Z., M.A., G.V., P.G.S.J., L.P., D.P.-A. and G.O.-D.; software, M.F.-R. and G.O.-D.; validation, M.F.-R., S.P., M.S.-R., A.E.-Z., M.A., G.V., P.G.S.J., L.P., D.P.-A. and G.O.-D.; formal analysis, M.F.-R., S.P. and G.O.-D.; investigation, M.F.-R., S.P., M.S.-R., A.E.-Z., M.A., G.V., P.G.S.J., L.P., D.P.-A. and G.O.-D.; resources, L.P., D.P.-A. and G.O.-D.; data curation, M.F.-R., S.P., M.S.-R., A.E.-Z., M.A., G.V., P.G.S.J., L.P., D.P.-A. and G.O.-D.; writing, original draft preparation, M.F.-R., S.P. and G.O.-D.; writing, review and editing, M.F.-R., S.P., M.S.-R., A.E.-Z., M.A., G.V., P.G.S.J., L.P., D.P.-A. and G.O.-D.; visualization, L.P., D.P.-A. and G.O.-D.; supervision, L.P., D.P.-A. and G.O.-D.; project administration, L.P., D.P.-A. and G.O.-D. All authors have read and agreed to the published version of the manuscript DS Docta Complutense RD 31 dic 2025