RT Journal Article
T1 First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6
A1 Civera, Alba
A1 Tvorynska, Sofiia
A1 Gamella Carballo, María
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Pedrero Muñoz, María
A1 Galan-Malo, Patricia
A1 Mata, Luis
A1 Sanchez, Lourdes
A1 Barek, Jiri
A1 Pingarrón Carrazón, José Manuel
A1 Campuzano Ruiz, Susana
A1 Perez, Maria D.
AB A novel electrochemical bioplatform for Pru du 6 determination, one of the most abundant and allergenic almond proteins, was developed to detect traces of almonds in processed foods. The bioplatform combines the application of a sandwich immunoassay on the surface of magnetic beads using specific antibodies unmodified or conjugated to horseradish peroxidase as capture and detection antibodies. The resulting magnetic bioconjugates are trapped on the surface of disposable carbon electrodes, and amperometry in the presence of hydrogen peroxide and hydroquinone is employed to measure the change in the cathodic current, which is proportional to Pru du 6 concentration. The developed bioplatform exhibited good selectivity and sensitivity, offering a detection limit of 0.12 ng/mL of Pru du 6. It could determine Pru du 6 in raw dough and baked cookies incurred with 0.2 µg/ g of almond protein with acceptable precision, providing results comparable to those obtained using an ELISA technique.
PB Elsevier
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/114295
UL https://hdl.handle.net/20.500.14352/114295
LA eng
NO Alba Civera, Sofiia Tvorynska, Maria Gamella, Rebeca M. Torrente-Rodríguez, María Pedrero, Patricia Galán-Malo, Luis Mata, Lourdes Sánchez, Jiří Barek, José M. Pingarrón, Susana Campuzano, María D. Pérez, First biosensing platform for detecting traces of almonds in processed foods by electrochemical determination of the allergenic protein Pru du 6, Microchemical Journal, Volume 195, 2023, 109403
DS Docta Complutense
RD 22 mar 2026