RT Journal Article
T1 Monitoring membrane viscosity in differentiating stem cells using BODIPY-based molecular rotors and FLIM
A1 Kashirina, Alena S.
A1 López Duarte, Ismael
A1 Kubánková, Markéta
A1 Gulin, Alexander A.
A1 Dudenkova, Varvara V.
A1 Rodimova, Svetlana A.
A1 Torgomyan, Hayk G.
A1 Zagaynova, Elena V.
A1 Meleshina, Aleksandra V.
A1 Kuimova, Marina K.
AB Membrane fluidity plays an important role in many cell functions such as cell adhesion, and migration. In stem cell lines membrane fluidity may play a role in differentiation. Here we report the use of viscosity-sensitive fluorophores based on a BODIPY core, termed “molecular rotors”, in combination with Fluorescence Lifetime Imaging Microscopy, for monitoring of plasma membrane viscosity changes in mesenchymal stem cells (MSCs) during osteogenic and chondrogenic differentiation. In order to correlate the viscosity values with membrane lipid composition, the detailed analysis of the corresponding membrane lipid composition of differentiated cells was performed by time-of-flight secondary ion mass spectrometry. Our results directly demonstrate for the first time that differentiation of MSCs results in distinct membrane viscosities, that reflect the change in lipidome of the cells following differentiation.
PB Springer Science and Business Media LLC
SN 2045-2322
YR 2020
FD 2020-08-20
LK https://hdl.handle.net/20.500.14352/115339
UL https://hdl.handle.net/20.500.14352/115339
LA eng
NO Kashirina AS, López-Duarte I, Kubánková M, Gulin AA, Dudenkova VV, Rodimova SA, et al. Monitoring membrane viscosity in differentiating stem cells using BODIPY-based molecular rotors and FLIM. Sci Rep [Internet]. 20 de agosto de 2020 [citado 21 de enero de 2025];10(1):14063. Disponible en: https://www.nature.com/articles/s41598-020-70972-5
NO Russian Science Foundation
NO Russian Foundation for Basic Research
DS Docta Complutense
RD 10 abr 2025