RT Journal Article
T1 Early Diversification of Membrane Intrinsic Proteins (MIPs) in Eukaryotes
A1 Irisarri, Iker
A1 Lorente Martínez, Héctor
A1 Strassert, Jürgen F.H.
A1 Agorreta, Ainhoa
A1 Zardoya, Rafael
A1 San Mauro Martín, Diego
A1 de Vries, Jan
AB Membrane intrinsic proteins (MIPs), including aquaporins (AQPs) and aquaglyceroporins (GLPs), form an ancient family of transporters for water and small solutes across biological membranes. The evolutionary history and functions of MIPs have been extensively studied in vertebrates and land plants, but their widespread presence across the eukaryotic tree of life suggests both a more complex evolutionary history and a broader set of functions than previously thought. That said, the early evolution of MIPs remains obscure. The presence of one GLP and four AQP clades across both bacteria and archaea suggests that the first eukaryotes could have possessed up to five MIPs. Here, we report on a previously unknown richness in MIP diversity across all major eukaryotic lineages, including unicellular eukaryotes, which make up the bulk of eukaryotic diversity. Three MIP clades have likely deep evolutionary origins, dating back to the last eukaryotic common ancestor (LECA), and support the presence of a complex MIP repertoire in early eukaryotes. Overall, our findings highlight the growing complexity of the reconstructed LECA genome: the dynamic evolutionary history of MIPs was set in motion when eukaryotes were in their infancy followed by radiative bursts across all main eukaryotic lineages.
PB Oxford University Press
SN 1759-6653
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/117608
UL https://hdl.handle.net/20.500.14352/117608
LA eng
NO Irisarri, I., Lorente-Martínez, H., Strassert, J. F. H., Agorreta, A., Zardoya, R., San Mauro, D., & De Vries, J. (2024). Early diversification of membrane intrinsic proteins (Mips) in eukaryotes. Genome Biology and Evolution, 16(8), evae164. https://doi.org/10.1093/gbe/evae164
NO H.L-M. was sponsored by a predoctoral contract of the Complutense University of Madrid and the Real Colegio Complutense at Harvard University (RCC-UCM), and an ERASMUS + research mobility traineeship from the European Commission. J.d.V. thanks the European Research Council for funding under the European Union's Horizon 2020 programme (Grant Agreement No. 852725; ERC-StG “TerreStriAL”) and the German Research Foundation (DFG) within the framework of the Priority Programme “MAdLand—Molecular Adaptation to Land: Plant Evolution to Change” (SPP 2237; VR 132/4-1), in which I.I. partakes as an associate member. J.F.H.S. acknowledges support from the German Research Foundation (DFG; grant STR1349/2-1, project no. 432453260). We acknowledge support by the Open Access Publication Funds of the University of Goettingen.
NO Universidad Complutense de Madrid
NO European Commission
NO German Research Foundation
NO University of Goettingen
DS Docta Complutense
RD 9 abr 2025