RT Journal Article
T1 Antimicrobial Activity of EPA and DHA against Oral Pathogenic Bacteria Using an In Vitro Multi-Species Subgingival Biofilm Model
A1 Ribeiro Vidal, Honorato
A1 Sánchez Beltrán, María Del Carmen
A1 Alonso Español, Andrea
A1 Figuero Ruiz, Elena
A1 Ciudad Cabañas, María José
A1 Collado Yurrita, Luis Rodolfo
A1 Herrera González, David
A1 Sanz Alonso, Mariano
AB In search for natural products with antimicrobial properties for use in the prevention and treatment of periodontitis, the purpose of this investigation was to evaluate the antimicrobial activity of two omega-3 fatty acids, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), using an in vitro multi-species subgingival biofilm model including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of EPA and DHA extracts (100 µM) and the respective controls were assessed on 72 h biofilms by their submersion onto discs for 60 s. Antimicrobial activity was evaluated by quantitative polymerase chain reaction (qPCR), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). ANOVA with Bonferroni correction was used to evaluate the antimicrobial activity of each of the fatty acids. Both DHA and EPA significantly reduced (p < 0.001 in all cases) the bacterial strains used in this biofilm model. The results with CLSM were consistent with those reported with qPCR. Structural damage was evidenced by SEM in some of the observed bacteria. It was concluded that both DHA and EPA have significant antimicrobial activity against the six bacterial species included in this biofilm model.
PB MDPI
YR 2020
FD 2020-09-14
LK https://hdl.handle.net/20.500.14352/109226
UL https://hdl.handle.net/20.500.14352/109226
LA eng
NO Ribeiro-Vidal H, Sánchez MC, Alonso-Español A, Figuero E, Ciudad MJ, Collado L, Herrera D, Sanz M. Antimicrobial Activity of EPA and DHA against Oral Pathogenic Bacteria Using an In Vitro Multi-Species Subgingival Biofilm Model. Nutrients. 2020 Sep 14;12(9):2812. doi: 10.3390/nu12092812
DS Docta Complutense
RD 9 abr 2025