RT Journal Article
T1 Synthesis, physicochemical characterization and biological evaluation of chitosan sulfate as heparan sulfate mimics
A1 Doncel Pérez, Ernesto
A1 Aranaz Corral, Inmaculada
A1 Acosta Contreras, Florentina Niuris
A1 Civera Tejuca, María Concepción
A1 Bastida Codina, Agatha
A1 Revuelta Crespo, Julia
A1 Camacho Toledano, Celia
A1 Garrido Fernández, Leoncio
A1 García Junceda, Eduardo
A1 Fernández Mayoralas, Alfonso
A1 Heras Caballero, Ángeles María
AB Despite the relevant biological functions of heparan sulfate (HS) glycosaminoglycans, their limited availability and the chemical heterogeneity from natural sources hamper their use for biomedical applications. Chitosan sulfates (ChS) exhibit structural similarity to HSs and may mimic their biological functions. We prepared a variety of ChS with different degree of sulfation to evaluate their ability to mimic HS in protein binding and to promote neural cell division and differentiation. The structure of the products was characterized using various spectroscopic and analytical methods. The study of their interaction with different growth factors showed that ChS bound to the proteins similarly or even better than heparin. In cell cultures, a transition effect on cell number was observed as a function of ChS concentration. Differences in promoting the expression of the differentiation markers were also found depending on the degree of sulfation and modification in the chitosan.
SN 0144-8617
YR 2018
FD 2018-07
LK https://hdl.handle.net/20.500.14352/96541
UL https://hdl.handle.net/20.500.14352/96541
LA eng
NO Doncel-Pérez, Ernesto, et al. «Synthesis, Physicochemical Characterization and Biological Evaluation of Chitosan Sulfate as Heparan Sulfate Mimics». Carbohydrate Polymers, vol. 191, julio de 2018, pp. 225-33. DOI.org (Crossref), https://doi.org/10.1016/j.carbpol.2018.03.036.
NO Ministerio de Economía, Comercio y Empresa (España)
DS Docta Complutense
RD 4 abr 2025