RT Journal Article T1 Biotin-functionalized nanoparticles: an overview of recent trends in cancer detection A1 Fathi Karkan, Sonia A1 Sargazi, Saman A1 Shojaei, Shirin A1 Farasati, Bahareh A1 Mirinejad, Shekoufeh A1 Cordani, Marco A1 Khosravi, Arezoo A1 Zarrabi, Ali A1 Ghavami, Saeid AB Electrochemical bio-sensing is a potent and efficient method for converting various biological recognition events into voltage, current, and impedance electrical signals. Biochemical sensors are now a common part of medical applications, such as detecting blood glucose levels, detecting food pathogens, and detecting specific cancers. As an exciting feature, bio-affinity couples, such as proteins with aptamers, ligands, paired nucleotides, and antibodies with antigens, are commonly used as bio-sensitive elements in electrochemical biosensors. Biotin-avidin interactions have been utilized for various purposes in recent years, such as targeting drugs, diagnosing clinically, labeling immunologically, biotechnology, biomedical engineering, and separating or purifying biomolecular compounds. The interaction between biotin and avidin is widely regarded as one of the most robust and reliable noncovalent interactions due to its high bi-affinity and ability to remain selective and accurate under various reaction conditions and bio-molecular attachments. More recently, there have been numerous attempts to develop electrochemical sensors to sense circulating cancer cells and the measurement of intracellular levels of protein thiols, formaldehyde, vitamin-targeted polymers, huwentoxin-I, anti-human antibodies, and a variety of tumor markers (including alpha-fetoprotein, epidermal growth factor receptor, prostate-specific Ag, carcinoembryonic Ag, cancer antigen 125, cancer antigen 15-3, etc.). Still, the non-specific binding of biotin to endogenous biotin-binding proteins present in biological samples can result in false-positive signals and hinder the accurate detection of cancer biomarkers. This review summarizes various categories of biotin-functional nanoparticles designed to detect such biomarkers and highlights some challenges in using them as diagnostic tools. PB Royal Society of Chemistry SN 2040-3364 YR 2024 FD 2024-06-10 LK https://hdl.handle.net/20.500.14352/106981 UL https://hdl.handle.net/20.500.14352/106981 LA eng NO S. Fathi-karkan, S. Sargazi, S. Shojaei, B. Farasati Far, S. Mirinejad, M. Cordani, A. Khosravi, A. Zarrabi and S. Ghavami, Nanoscale, 2024, 16, 12750 DOI: 10.1039/D4NR00634h NO Marco Cordani recibió el apoyo de la subvención RYC2021-031003I financiada por MICIU/AEI/10.13039/501100011033 y por la Unión Europea NextGenerationEU/PRTR. NO European Commission NO Ministerio de Ciencia, Innovación y Universidades (España) DS Docta Complutense RD 15 abr 2025