RT Journal Article
T1 Value of high-performance liquid chromatographic analysis of amino acids in the determination of Panax ginseng radix extract effect in cultured neurons
A1 Naval López, María Victoria
A1 Gómez-Serranillos Cuadrado, María Pilar
A1 Carretero Accame, María Emilia
A1 Arce De Obieta, Carmen
AB The present research describes a reversed-phase high-performance liquid chromatographic (RP-HPLC) method that allows the determination of several amino acids in primary cultured cortical neurons of rats. The concentration of amino acids was determined by using pre-column derivatization with dansyl chloride and UV-diode array detection. Data show that Panax ginseng radix extract (GS) can modulate amino acid release in neurons. The levels of glutamate (Glu), aspartate (Asp), γ-aminobutyric acid (GABA) and glycine (Gly) in the GS-treated groups were higher than in the non-treated groups dose-dependentwise. In this case, Glu and GABA were the most released amino acids (74.43% ± 0.97 and 88.41% ± 4.12 at ginseng dose 0.01 mg/ml after 1 h from treatment, respectively). The values obtained in the determination of the analytical parameters (linearity, precision, limit of detection and accuracy) confirm the quality of the method. The average recoveries for intra and inter-day assay (n = 5) were 101.18 and 102.38 for Asp, 99.35 and 98.44 for Glu, 99.59 and 99.66 for Gly, and 100.06 and 100.37 for GABA. These data proved that the method yields accurate results, with RSD lower than 2.2%. The precision of the method was estimated on the basis of RSD of six injections at two different concentrations of amino acids. This technique is useful in studying the GS-mediated modulation of the dynamic equilibrium of amino acids and neurotransmission in neurons.
PB Elsevier
SN 0021-9673
YR 2006
FD 2006
LK https://hdl.handle.net/20.500.14352/117154
UL https://hdl.handle.net/20.500.14352/117154
LA eng
NO Naval, M. V., et al. «Value of High-Performance Liquid Chromatographic Analysis of Amino Acids in the Determination of Panax Ginseng Radix Extract Effect in Cultured Neurons». Journal of Chromatography A, vol. 1121, n.o 2, julio de 2006, pp. 242-47. DOI.org (Crossref), https://doi.org/10.1016/j.chroma.2006.04.051
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 9 abr 2025