RT Journal Article
T1 Haemoglobin, a new major allergen of Anisakis simplex
T2 La hemoglobina, un nuevo alergeno principal de Anisakis simplex
A1 Daschner, Alvaro
A1 Nieuwenhuizen, Natalie E.
A1 Lopata, Andreas L.
A1 Frutos, Consolación De
A1 Valls, Ana
A1 González Fernández, Juan
A1 Cuéllar Del Hoyo, María Del Carmen
AB Gastro-allergic anisakiasis and Anisakis sensitisation associated chronic urticaria are diseases which differ in their IgE and IgG4 responses against both crude extract and specific allergens. Anisakis and Ascaris are closely related nematodes that usually cause problems with specificity in immunodiagnostics. In this study we measured IgE and IgG4 antibodies against Anisakis simplex sensu lato (s. l.) and Ascaris suum haemoglobins in sera of 21 gastro-allergic anisakiasis and 23 chronic urticaria patients. We used a capture ELISA with the anti-Anisakis haemoglobin monoclonal antibody 4E8g, which also recognises Ascaris haemoglobin. In addition, we determined specific IgE and IgG4 to both nematodes by indirect ELISA and immunoblotting. Anti-A. simplex s. l. haemoglobin IgE and IgG4 levels were higher in gastro-allergic anisakiasis than in chronic urticaria patients (P=0.002 and 0.026, respectively). Surprisingly, no patient had detectable IgE levels against A. suum haemoglobin. Finally, we carried out an in silico study of the B-cell epitopes of both haemoglobin molecules. Five epitopes were predicted in Anisakis pegreffii and four in A. suum haemoglobin. The epitope propensity values of Anisakis haemoglobin in the equivalent IgE binding region of the allergenic haemoglobin Chi t 1 from Chironomus thummi, were higher those of the Ascaris haemoglobin. In conclusion, we describe A. simplex haemoglobin as a new major allergen (Ani s 13), being recognised by a large number (64.3%) of sensitised patients and up to 80.9% in patients with gastro-allergic anisakiasis. The presence of a specific epitope and the different values of epitope propensity between Anisakis and Ascaris haemoglobin could explain the lack of cross-reactivity between the two molecules. The absence of IgE reactivity to Ascaris haemoglobin in Anisakis patients makes Anisakis haemoglobin (Ani s 13) a potential candidate for developing more specific diagnosis tools.
PB Elsevier
SN 0020-7519
YR 2015
FD 2015-05
LK https://hdl.handle.net/20.500.14352/94037
UL https://hdl.handle.net/20.500.14352/94037
LA eng
NO González-Fernández J, Daschner A, Nieuwenhuizen NE, Lopata AL, Frutos CD, Valls A, Cuéllar C. Haemoglobin, a new major allergen of Anisakis simplex. Int J Parasitol. 2015 May;45(6):399-407.
NO Mutua Madrileña
NO Sociedad Española de Alergología e Inmunología Clínica
NO Fundación Ramón Areces
NO Allergy Society of South Africa
NO Universidad Complutense de Madrid
NO Australian Research Council
DS Docta Complutense
RD 29 abr 2025