RT Journal Article T1 Pre‐ and post‐junctional bradykinin B2 receptors regulate smooth muscle tension to the pig intravesical ureter A1 Fernandes Ribeiro, Ana Sofía A1 Fernandes, Vítor S. A1 Martínez Sainz, María Del Pilar A1 López-Oliva Muñoz, María Elvira A1 Barahona Gomáriz, María Victoria A1 Recio Visedo, María Paz A1 Martínez Gómez, Ana Cristina A1 Blaha, Igor A1 Orensanz Muñoz, Luis Miguel A1 Bustamante, Salvador A1 García Sacristán, Albino A1 Prieto Ocejo, Dolores A1 Hernández Rodríguez, Medardo Vicente AB AimsNeuronal and non-neuronal bradykinin (BK) receptors regulate the contractility of the bladder urine outflow region. The current study investigates the role of BK receptors in the regulation of the smooth muscle contractility of the pig intravesical ureter.MethodsWestern blot and immunohistochemistry were used to show the expression of BK B1 and B2 receptors and myographs for isometric force recordings.ResultsB2 receptor expression was consistently detected in the intravesical ureter urothelium and smooth muscle layer, B1 expression was not detected where a strong B2 immunoreactivity was observed within nerve fibers among smooth muscle bundles. On ureteral strips basal tone, BK induced concentration-dependent contractions, were potently reduced by extracellular Ca2+ removal and by B2 receptor and voltage-gated Ca2+ (VOC) channel blockade. BK contraction did not change as a consequence of urothelium mechanical removal or cyclooxygenase and Rho-associated protein kinase inhibition. On 9,11-dideoxy-9a,11a-methanoepoxy prostaglandin F2α (U46619)-precontracted samples, under non-adrenergic non-cholinergic (NANC) and nitric oxide (NO)-independent NANC conditions, electrical field stimulation-elicited frequency-dependent relaxations which were reduced by B2 receptor blockade. Kallidin, a B1 receptor agonist, failed to increase preparation basal tension or to induce relaxation on U46619-induced tone.ConclusionsThe present results suggest that BK produces contraction of pig intravesical ureter via smooth muscle B2 receptors coupled to extracellular Ca2+ entry mainly via VOC (L-type) channels. Facilitatory neuronal B2 receptors modulating NO-dependent or independent NANC inhibitory neurotransmission are also demonstrated. Neurourol. Urodynam. 35:115–121, 2016. © 2014 Wiley Periodicals, Inc. PB Wiley SN 0733-2467 SN 1520-6777 YR 2016 FD 2016-01-01 LK https://hdl.handle.net/20.500.14352/97696 UL https://hdl.handle.net/20.500.14352/97696 LA eng NO Ribeiro AS, Fernandes VS, Martínez MP, et al. Pre- and post-junctional bradykinin B2 receptors regulate smooth muscle tension to the pig intravesical ureter. Neurourol Urodyn. 2016;35(1):115-121. doi:10.1002/nau.22685 DS Docta Complutense RD 23 jul 2024