RT Journal Article
T1 Mesoporous Silica Nanoparticles as a Potential Platform for Vaccine Development against Tuberculosis
A1 Montalvo Quirós, Sandra
A1 Vallet Regí, María Dulce Nombre
A1 Palacios, Ainhoa
A1 Anguita, Juan
A1 Prados-Rosales, Rafael C.
A1 González Ortiz, Blanca
A1 Luque García, José Luis
AB The increasing emergence of new strains of Mycobacterium tuberculosis (Mtb) highly resistant to antibiotics constitute a public health issue, since tuberculosis still constitutes the primary cause of death in the world due to bacterial infection. Mtb has been shown to produce membrane-derived extracellular vesicles (EVs) containing proteins responsible for modulating the pathological immune response after infection. These natural vesicles were considered a promising alternative to the development of novel vaccines. However, their use was compromised by the observed lack of reproducibility between preparations. In this work, with the aim of developing nanosystems mimicking the extracellular vesicles produced by Mtb, mesoporous silica nanoparticles (MSNs) have been used as nanocarriers of immunomodulatory and vesicle-associated proteins (Ag85B, LprG and LprA). These novel nanosystems have been designed and extensively characterized, demonstrating the effectiveness of the covalent anchorage of the immunomodulatory proteins to the surface of the MSNs. The immunostimulatory capacity of the designed nanosystems has been demonstrated by measuring the levels of pro- (TNF) and anti-inflammatory (IL-10) cytokines in exposed macrophages. These results open a new possibility for the development of more complex nanosystems, including additional vesicle components or even antitubercular drugs, thus allowing for the combination of immunomodulatory and bactericidal effects against Mtb.
PB MDPI
SN 1999-4923
YR 2020
FD 2020-12-16
LK https://hdl.handle.net/20.500.14352/105861
UL https://hdl.handle.net/20.500.14352/105861
LA eng
NO Montalvo-Quirós, S.; Vallet-Regí, M.; Palacios, A.; Anguita, J.; Prados-Rosales, R.C.; González, B.; Luque-Garcia, J.L. Mesoporous Silica Nanoparticles as a Potential Platform for Vaccine Development against Tuberculosis. Pharmaceutics 2020, 12, 1218. https://doi.org/10.3390/pharmaceutics12121218
DS Docta Complutense
RD 7 oct 2024