RT Journal Article
T1 Novel blaROB-1-bearing plasmid conferring resistance to β-lactams in Haemophilus parasuis isolates from healthy weaning pigs
A1 Moleres, Javier
A1 Santos López, Alfonso
A1 Lázaro, Isidro
A1 Labairu, Javier
A1 Prat, Cristina
A1 Ardanuy, Carmen
A1 González Zorn, Bruno
A1 Aragón, Virginia
A1 Garmendia, Junkal
AB Haemophilus parasuis, the causative agent of Glässer's disease, is one of the early colonizers of the nasal mucosa of piglets. It is prevalent in swine herds, and lesions associated with disease are fibrinous polyserositis and bronchopneumonia. Antibiotics are commonly used in disease control, and resistance to several antibiotics has been described in H. parasuis. Prediction of H. parasuis virulence is currently limited by our scarce understanding of its pathogenicity. Some genes have been associated with H. parasuis virulence, such as lsgB and group 1 vtaA, while biofilm growth has been associated with nonvirulent strains. In this study, 86 H. parasuis nasal isolates from farms that had not had a case of disease for more than 10 years were obtained by sampling piglets at weaning. Isolates were studied by enterobacterial repetitive intergenic consensus PCR and determination of the presence of lsgB and group 1 vtaA, biofilm formation, inflammatory cell response, and resistance to antibiotics. As part of the diversity encountered, a novel 2,661-bp plasmid, named pJMA-1, bearing the blaROB-1 β-lactamase was detected in eight colonizing strains. pJMA-1 was shown to share a backbone with other small plasmids described in the Pasteurellaceae, to be 100% stable, and to have a lower biological cost than the previously described plasmid pB1000. pJMA-1 was also found in nine H. parasuis nasal strains from a separate collection, but it was not detected in isolates from the lesions of animals with Glässer's disease or in nontypeable Haemophilus influenzae isolates. Altogether, we show that commensal H. parasuis isolates represent a reservoir of β-lactam resistance genes which can be transferred to pathogens or other bacteria.
PB American Society for Microbiology
SN 1098-5336
YR 2015
FD 2015-05-01
LK https://hdl.handle.net/20.500.14352/24677
UL https://hdl.handle.net/20.500.14352/24677
LA eng
NO Unión Europea. FP7
NO Ministerio de Economía y Competitividad (MINECO)
NO Gobierno de Navarra
DS Docta Complutense
RD 16 may 2024