RT Journal Article
T1 The Fusarium oxysporum cell wall proteome under adhesion-inducing conditions
A1 Prados-Rosales, Rafael
A1 Luque García, José Luis
A1 Martínez López, Raquel María
A1 Gil García, Concepción
A1 Di Pietro, Antonio
AB Fusarium oxysporum is a soilborne fungus that causes vascular wilt disease on a wide range of crops. During initial stages of infection, fungal hyphae attach firmly to roots, penetrate the cortex and colonize xylem vessels. The mechanisms underlying root attachment are poorly understood, although it was previously shown that this process depends on Fmk1, a mitogenactivated protein kinase orthologous to the mating/filamentation mitogen-activated protein kinases Fus3/Kss1 in yeast. We investigated the hypothesis that root adhesion is mediated by fungal cell wall proteins (CWPs). To characterize the cell wall subproteome of F. oxysporum, we performed LC-MS/MS analysis of tryptic digests of purified cell walls obtained from adhesion-inducing conditions, identifying a total of 174 proteins, 19 of which contain apredicted signal peptide and 10 of which have a predicted glycosylphosphatidyl-inositol motif. 2-D DIGE was used to compare four different fractions of CWPs extracted from hyphae of the wild-type strain and the Dfmk1 mutant. We detected 18 proteins differing significantly in abundance between the two strains. Differential expression of five of these proteins was confirmed by RT-PCR analysis. A significant fraction of the subproteome lacked functional information, highlighting the limitations in the current understanding of CWPs in F. oxysporum.
SN 1615-9853
YR 2009
FD 2009-10-09
LK https://hdl.handle.net/20.500.14352/95554
UL https://hdl.handle.net/20.500.14352/95554
LA eng
NO Prados‐Rosales R, Luque‐Garcia JL, Martínez‐López R, Gil C, Di Pietro A. The Fusarium oxysporum cell wall proteome under adhesion‐inducing conditions. Proteomics 2009;9:4755–69. https://doi.org/10.1002/pmic.200800950.
DS Docta Complutense
RD 10 abr 2025