%0 Journal Article
%A Clemente, Luis Felipe
%A Hernáez, María Luisa
%A Ramos-Fernández, Antonio
%A Ligero, Gertrudis
%A Gil, Concha
%A Corrales, Fernando José
%A Marcilla, Miguel
%T Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.
%D 2018
%@ 1535-3907
%U https://hdl.handle.net/20.500.14352/13379
%X Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.
%~