RT Journal Article
T1 Identification of the Missing Protein Hyaluronan Synthase 1 in Human Mesenchymal Stem Cells Derived from Adipose Tissue or Umbilical Cord.
A1 Clemente, Luis Felipe
A1 Hernáez, María Luisa
A1 Ramos-Fernández, Antonio
A1 Ligero, Gertrudis
A1 Gil, Concha
A1 Corrales, Fernando José
A1 Marcilla, Miguel
AB Currently, 14% of the human proteome is made up of proteins whose existence is not confirmed by mass spectrometry. We performed a proteomic profiling of human mesenchymal stem cells derived from adipose tissue or umbilical cord (PRIDE accession number: PXD009893) and identified peptides derived from 13 of such missing proteins. Remarkably, we found compelling evidence of the expression of hyaluronan synthase 1 (NX_Q92839-1) and confirmed its identification by the fragmentation of four heavy-labeled peptides that coeluted with their endogenous light counterparts. Our data also suggest that mesenchymal stem cells constitute a promising source for the detection of missing proteins.
PB American Chemical Society
SN 1535-3907
YR 2018
FD 2018-07-05
LK https://hdl.handle.net/20.500.14352/13379
UL https://hdl.handle.net/20.500.14352/13379
LA eng
NO ISCIII and ERDF
NO BNLC-ISCIII
DS Docta Complutense
RD 4 may 2024