RT Journal Article
T1 Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador
A1 David Ortega-Paredes, 
A1 Sofía de Janon, 
A1 Fernando Villavicencio, 
A1 Katherine Jaramillo Ruales, 
A1 Kenny De La Torre, 
A1 José E. Villacís, 
A1 Jaap A. Wagenaar, 
A1 Jorge Matheu, 
A1 Camila Bravo-Vallejo, 
A1 Esteban Fernández-Moreira, 
A1 Christian Vinueza-Burgos, 
AB Antimicrobial resistance (AMR) is a major health threat for public and animal healthin the twenty-first century. In Ecuador, antibiotics have been used by the poultryindustry for decades resulting in the presence of multi-drug resistant (MDR) bacteriain the poultry meat production chain, with the consequent risk for public health. Thisstudy evaluated the prevalence of ESBL/AmpC and mcr genes in third-generationcephalosporin-resistant Escherichia coli (3GC-R E. coli) isolated from broiler farms(animal component), broiler carcasses (food component), and human enteritis (humancomponent) in Quito-Ecuador. Samples were collected weekly from November 2017to November 2018. For the animal, food, and human components, 133, 335, and 302samples were analyzed, respectively. Profiles of antimicrobial resistance were analyzedby an automated microdilution system. Resistance genes were studied by PCR andSanger sequencing. From all samples, 122 (91.7%), 258 (77%), and 146 (48.3%)samples were positive for 3GC-R E. coli in the animal, food, and human components,respectively. Most of the isolates (472/526, 89.7%) presented MDR phenotypes. TheESBL blaCTX-M-55, blaCTX-M-3, blaCTX-M-15, blaCTX-M-65, blaCTX-M-27, and blaCTX-M-14were the most prevalent ESBL genes while blaCMY-2 was the only AmpC detected gene.The mcr-1 gene was found in 20 (16.4%), 26 (10.1%), and 3 (2.1%) of isolates fromanimal, food, and human components, respectively. The implication of poultry productsin the prevalence of ESBL/AmpC and mcr genes in 3GC-R must be considered in thesurveillance of antimicrobial resistance.
PB Frontiers
YR 2020
FD 2020-11-25
LK https://hdl.handle.net/20.500.14352/124980
UL https://hdl.handle.net/20.500.14352/124980
LA eng
NO Ortega-Paredes, David, et al. «Broiler Farms and Carcasses Are an Important Reservoir of Multi-Drug Resistant Escherichia coli in Ecuador». Frontiers in Veterinary Science, vol. 7, noviembre de 2020, p. 547843. DOI.org (Crossref), https://doi.org/10.3389/fvets.2020.547843.
DS Docta Complutense
RD 30 mar 2026