RT Journal Article
T1 Calcium oscillations in human mesenteric vascular smooth muscle
A1 Navarro Dorado, Jorge
A1 García Alonso, Mauricio
A1 Van Breemen, Casey
A1 Tejerina Sánchez, María Teresa
A1 Fameli, Nicola
AB Phenylephrine (PE)-induced oscillatory fluctuations in intracellular Ca2+ concentration ([Ca2+]i) of vascular smooth muscle have been observed in many blood vessels isolated from a wide variety of mammals. Paradoxically, until recently similar observations in humans have proven elusive. In this study, we report for the first time observations of adrenergically-stimulated [Ca2+]i oscillations in human mesenteric artery smooth muscle. In arterial segments preloaded with Fluo-4 AM and mounted on a myograph on the stage of a confocal microscope, we observed PE-induced oscillations in [Ca2+]i, which initiated and maintained vasoconstriction. These oscillations present some variability, possibly due to compromised health of the tissue. This view is corroborated by our ultrastructural analysis of the cells, in which we found only (5 ± 2)% plasma membrane-sarcoplasmic reticulum apposition, markedly less than measured in healthy tissue from laboratory animals. We also partially characterized the oscillations by using the inhibitory drugs 2-aminoethoxydiphenyl borate (2-APB), cyclopiazonic acid (CPA) and nifedipine. After PE contraction, all drugs provoked relaxation of the vessel segments, sometimes only partial, and reduced or inhibited oscillations, except CPA, which rarely caused relaxation. These preliminary results point to a potential involvement of the sarcoplasmic reticulum Ca2+ and inositol 1,4,5-trisphosphate receptor (IP3R) in the maintenance of the Ca2+ oscillations observed in human blood vessels.
SN 0006-291X
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/115757
UL https://hdl.handle.net/20.500.14352/115757
LA eng
NO : J. Navarro-Dorado et al., Calcium oscillations in human mesenteric vascular smooth muscle, Biochem. Biophys. Res. Commun. (2014), http://dx.doi.org/10.1016/j.bbrc.2014.01.150
NO Fondo de Investigaciones Sanitarias
NO Red Temática de Investigación Cardiovascular
NO Canadian Institute for Health Research
DS Docta Complutense
RD 18 abr 2025