RT Journal Article
T1 Bacterial lipopolysaccharide forms aggregates with apolipoproteins in male and female rat brains after ethanol binges
A1 López Valencia, Leticia
A1 Moya Montes, Marta
A1 Escudero Moreno, Berta
A1 García Bueno, Borja
A1 Orio Ortiz, Laura
AB Alcohol binge drinking allows the translocation of bacterial lipopolysaccharide (LPS) from the gut to the blood, which activates the peripheral immune system with consequences in neuroinflammation. A possible access/direct signaling of LPS to/in the brain has not yet been described under alcohol abuse conditions. Apolipoproteins are compounds altered by alcohol with high affinity to LPS which may be involved in its transport to the brain or in its elimination. Here, we explored the expression of small components of LPS, in its free form or bound to apolipoproteins, in the brain of female and male rats exposed to alcohol binges. Animals received ethanol oral gavages (3 g/kg every 8 h) for 4 days. LPSor its components (Lipid A and core), LPS-binding protein, corticosterone, lipoproteins (HDL, LDL), apolipoproteins (ApoAI, ApoB, and ApoE), and their receptors were measured in plasma and/or in nonperfused prefrontal cortex (PFC) and cerebellum. Brain LipidA-apolipoprotein aggregates were determined by Western blotting and confirmed by coimmunoprecipitation. In animals exposed to alcohol binges: 1) plasma LPS-binding protein was elevated in both sexes; 2) females showed elevations in plasma ApoAI and corticosterone levels; 3) Lipid A formed aggregates with ApoAI in the female PFC and with ApoB in males, the latter showing Toll-like receptor 4 upregulation in PFC but not females. These results suggest that small bacterial components are present within the brain, forming aggregates with different apolipoproteins, depending on the sex, after alcohol binge intoxications. Results may have implications for the crosstalk between alcohol, LPS, and neuroinflammation.
PB American Society for Biochemistry and Molecular Biology
YR 2024
FD 2024-01-29
LK https://hdl.handle.net/20.500.14352/101944
UL https://hdl.handle.net/20.500.14352/101944
LA eng
NO López-Valencia, L., Moya, M., Escudero, B., García-Bueno, B., & Orio, L. (2024). Bacterial lipopolysaccharide forms aggregates with apolipoproteins in male and female rat brains after ethanol binges. Journal of Lipid Research, 100509.
NO FEDER (European Union)
NO Ministerio de Ciencia, Innovación y Universidades (MICINN)
NO Agencia Estatal de Investigación (AEI)
DS Docta Complutense
RD 8 abr 2025