RT Journal Article
T1 Amperometric aptasensor with sandwich-type architecture for troponin I based on carboxyethylsilanetriol-modified graphene oxide coated electrodes
A1 Villalonga, Anabel
A1 Estabiel, Itziar
A1 Pérez-Calabuig, Ana
A1 Mayol Hornero, Beatriz
A1 Parrado Quintela, Concepción
A1 Villalonga Santana, Reynaldo
AB A novel amperometric aptasensor for the specific detection of cardiac troponin I (cTnI) was constructed by using screen-printed carbon electrodes coated with a carboxyethylsilanetriol-modified graphene oxide derivative as transduction element. This novel carboxylic acid-enriched nanomaterial allows easy and high load immobilization of the capture aptamer molecules on the electrode surface. The biosensing interface was assembled by covalent attachment of an amino-functionalized DNA aptamer on the carboxylic acid-enriched electrode surface. The sensing approach relies on the specific recognition of cTnI by the aptamer and further assembly of a sandwich-type architecture with a novel aptamer-peroxidase conjugate as signaling element. The aptasensor was employed to detect the cardiac biomarker in the broad range from 1.0 pg/mL to 1.0 μg/mL with a detection limit of 0.6 pg/mL. This electroanalytical device also showed high specificity, reproducibility and stability, and was useful to quantify cTnI in reconstituted human serum samples.
PB Elsevier B.V.
SN 0956-5663
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/99067
UL https://hdl.handle.net/20.500.14352/99067
LA eng
NO Villalonga, A., Estabiel, I., Pérez-Calabuig, A. M., Mayol, B., Parrado, C., Villalonga, R. (2021). Amperometric aptasensor with sandwich-type architecture for troponin I based on carboxyethylsilanetriol-modified graphene oxide coated electrodes. Biosensors and Bioelectronics 183: 113203.
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 9 abr 2025