RT Journal Article
T1 Hybrid Enzyme-Polymeric Capsules/Mesoporous Silica Nanodevice for In Situ Cytotoxic Agent Generation
A1 Baeza, Alejandro
A1 Guisasola, Eduardo
A1 Torres Pardo, María De La Almudena
A1 González Calbet, José María
A1 Melen, Gustavo J.
A1 Ramírez, Manuel
A1 Vallet Regí, María Dulce Nombre
AB A novel nanocarrier based on functionalized mesoporous silica nanoparticles able to transport a non-toxic pro-drug and the enzyme responsible for its activation is presented. This nanodevice is able to generate in situ cytotoxic species once accumulated in the tumoral cell. Enzymes are sensitive macromolecules which can suffer denaturalization in biological media due to the presence of proteases or other aggressive agents. Moreover, the direct attachment of enzymes to the silica surface can reduce their activity by conformational changes or active site blockage. For these reasons, in order to create a robust system able to work in living organisms, the enzymes are previously coated with a protective polymeric shell which allows the attachment on the silica surface preserving their activity. The efficacy of this hybrid nanodevice for antitumoral purposes is tested against several human tumoral cells as neuroblastoma and leukemia showing significant efficacy. It converts this device in a promising candidate for further in vivo studies for oncology therapy.
PB Wiley
SN 1616-301X
YR 2014
FD 2014-08-06
LK https://hdl.handle.net/20.500.14352/35409
UL https://hdl.handle.net/20.500.14352/35409
LA eng
NO Baeza, A., Guisasola, E., Torres Pardo, M. A. et al. «Hybrid Enzyme‐Polymeric Capsules/Mesoporous Silica Nanodevice for In Situ Cytotoxic Agent Generation». Advanced Functional Materials, vol. 24, n.o 29, agosto de 2014, pp. 4625-33. DOI.org (Crossref), https://doi.org/10.1002/adfm.201400729.
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)ORCID 0000-0002-9042-8865 (Alejandro Baeza)Researcher ID K-8193-2014  (Alejandro Baeza)Researcher ID 0000-0002-2549-1745 (Eduardo Guisasola Cal)Researcher ID E-8300-2012 (Eduardo Guisasola Cal)
NO Ministerio de Economía, Comercio y Empresa (España)
NO Comunidad de Madrid
NO Comisión Interministerial de Ciencia y Tecnología (España)
DS Docta Complutense
RD 11 abr 2025