RT Journal Article
T1 INF2 promotes the formation of detyrosinated microtubules necessary for centrosome reorientation in T cells
A1 Andrés-Delgado, Laura
A1 Antón Hurtado, Olga María
A1 Bartolini, Francesca
A1 Ruiz-Sáenz, Ana
A1 Correas, Isabel
A1 Gundersen, Gregg
A1 Alonso, Miguel
AB T cell antigen receptor–proximal signaling components, Rho-family GTPases, and formin proteins DIA1 and FMNL1 have been implicated in centrosome reorientation to the immunological synapse of T lymphocytes. However, the role of these molecules in the reorientation process is not yet defined. Here we find that a subset of microtubules became rapidly stabilized and that their α-tubulin subunit posttranslationally detyrosinated after engagement of the T cell receptor. Formation of stabilized, detyrosinated microtubules required the formin INF2, which was also found to be essential for centrosome reorientation, but it occurred independently of T cell receptor–induced massive tyrosine phosphorylation. The FH2 domain, which was mapped as the INF2 region involved in centrosome repositioning, was able to mediate the formation of stable, detyrosinated microtubules and to restore centrosome translocation in DIA1-, FMNL1-, Rac1-, and Cdc42-deficient cells. Further experiments indicated that microtubule stabilization was required for centrosome polarization. Our work identifies INF2 and stable, detyrosinated microtubules as central players in centrosome reorientation in T cells.
PB Rockefeller University Press
SN 1540-8140
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/97002
UL https://hdl.handle.net/20.500.14352/97002
LA eng
NO Laura Andrés-Delgado, Olga M. Antón, Francesca Bartolini, Ana Ruiz-Sáenz, Isabel Correas, Gregg G. Gundersen, Miguel A. Alonso; INF2 promotes the formation of detyrosinated microtubules necessary for centrosome reorientation in T cells. J Cell Biol 17 September 2012; 198 (6): 1025–1037. doi: https://doi.org/10.1083/jcb.201202137
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 18 abr 2025