RT Journal Article
T1 Threshold-dependent transcriptional discrimination underlies stem cell homeostasis
A1 Perales, Mariano
A1 Rodriguez, Kevin
A1 Snipes, Stephen
A1 Yadav, Ram Kishor
A1 Díaz Mendoza, María Mercedes
A1 Venugopala, Reddy
AB Transcriptional mechanisms that underlie the dose-dependent regulation of gene expression in animal development have been studied extensively. However, the mechanisms of dose-dependent transcriptional regulation in plant development have not been understood. In Arabidopsis shoot apical meristems, WUSCHEL (WUS), a stem cell-promoting transcription factor, accumulates at a higher level in the rib meristem and at a lower level in the central zone where it activates its own negative regulator, CLAVATA3 (CLV3). How WUS regulates CLV3 levels has not been understood. Here we show that WUS binds a group of cis-elements, cis- regulatory module, in the CLV3-regulatory region, with different affinities and conformations, consisting of monomers at lower concentration and as dimers at a higher level. By deleting cis elements, manipulating the WUS-binding affinity and the homodimerization threshold of cis elements, and manipulating WUS levels, we show that the same cis elements mediate both the activation and repression of CLV3 at lower and higher WUS levels, respectively. The concentration-dependent transcriptional discrimination provides a mechanistic framework to explain the regulation of CLV3 levels that is critical for stem cell homeostasis.
PB National Academy of Sciences
SN 0027-8424
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/95735
UL https://hdl.handle.net/20.500.14352/95735
LA eng
NO Perales, Mariano, et al. «Threshold-Dependent Transcriptional Discrimination Underlies Stem Cell Homeostasis». Proceedings of the National Academy of Sciences, vol. 113, n.o 41, octubre de 2016. https://doi.org/10.1073/pnas.1607669113.
NO National Science Foundation  
DS Docta Complutense
RD 6 abr 2025