RT Journal Article
T1 Effects of the HDAC inhibitor scriptaid on the in vitro development of bovine embryos and on imprinting gene expression levels
A1 Laguna Barraza, Ricardo
A1 Sánchez Calabuig, María Jesús
A1 Gutiérrez Adán, Alfonso
A1 Rizos, Dimitrios
A1 Perez Cerezales, Serafin
AB This study examines the effects of the histone deacetylation inhibitor scriptaid (SCR) on preimplantation embryo development in vitro and on imprinting gene expression. We hypothesized that SCR would increase histone acetylation levels, enhance embryonic genome activation, and regulate imprinting and X-chromosome inactivation (XCI) in in vitro produced bovine embryos. Zygotes were cultured in vitro in presence or absence of SCR added at different time points. We assessed cleavage and blastocyst rates as well as the quality of blastocysts through: (i) differential cell counts; (ii) survival after vitrification/thawing and (iii) gene expression analysis -including imprinted genes. Blastocyst yields were not different in the control and experimental groups. While no significant differences were observed between groups in total cell or trophectoderm cell numbers, SCR treatment reduced the number of inner cell mass cells and improved the survival of vitrified embryos. Further, genes involved in the mechanism of paternal imprinting (GRB10, GNAS, XIST) were downregulated in presence of SCR compared with controls. These observations suggest SCR prevents deacetylation of paternally imprinting control regions and/or their up-regulation, as these events took place in controls. Whether or not such reductions in XIST and imprinting gene expression are beneficial for post implantation development remains to be clarified.
PB Elsevier
SN 0093-691X
YR 2018
FD 2018-04-01
LK https://hdl.handle.net/20.500.14352/117845
UL https://hdl.handle.net/20.500.14352/117845
LA eng
NO Laguna-Barraza, Sánchez-Calabuig, Gutiérrez-Adán, Rizos, & Pérez-Cerezales. (2018). Effects of the HDAC inhibitor scriptaid on the in vitro development of bovine embryos and on imprinting gene expression levels. Theriogenology, 110, 79-85. https://doi.org/10.1016/J.THERIOGENOLOGY.2017.12.043
NO Ministerio de Economía, Comercio y Empresa (España)
DS Docta Complutense
RD 28 abr 2025